麻叶荨麻和羊草比例对体外培养瘤胃微生物发酵参数的影响  

Effects of the Ratio of Leymus chinensis and Urtica cannbinau on Rumen Microbial Fermentation in vitro

作　　者：王珊[1] 史良峰 张振斌[1] 王梦芝[1] 张晓庆[2] WANGH Shan;SHI Liangfeng;ZHANG Zhenbin;WANG Mengzhi;ZHANG Xiaoqing(College of Animal Science and Technology,Yangzhou University,Yangzhou Jiangsu 225009;Institute of Grassland Science,Chinese Academy of Agricultural Sciences,Hohhot Inner Mongolia 010010)

机构地区：[1]扬州大学动物科学与技术学院,江苏扬州225009 [2]中国农业科学院草原研究所,内蒙古呼和浩特010010

出　　处：《家畜生态学报》2018年第4期33-37,共5页Journal of Domestic Animal Ecology

基　　金：国家自然基金(31672446;31402119);江苏省苏北科技专项(富民强县:BN2016096)

摘　　要：试验旨在研究麻叶荨麻和羊草比例对体外培养瘤胃微生物发酵参数的影响。试验以底物中麻叶荨麻:羊草比例分5组:0∶100(A)、30∶70(B)、50∶50(C)、70∶30(D)、100∶0(E)进行瘤胃微生物体外培养。在培养后0、1、3、6、12、24h采集瘤胃液并检测其发酵参数。试验结果表明:(1)pH:1、3、6h的都以A组和C组的较高,并以C组的最高(P<0.05);(2)NH_3-N浓度:3、6、12、24h的以D组和E组的较高(P<0.05);(3)微生物蛋白(MCP):1、3、6、24h的MCP含量以A组和C组的较高(P<0.05),并以C组的最高(P<0.05);(4)产气量:0~1h、1~3h的以A组、B组和C组的较高(P<0.05),其中以C组的最高(P<0.05);(5)24hNDF降解率以C组的最高(P<0.05)。试验结果表明,底物中麻叶荨麻和羊草以50∶50比例的pH较高、氨氮浓度较低,同时该组的微生物蛋白含量、产气量和24h NDF降解率也较高,表明了该比例利于体外培养瘤胃微生物的发酵和底物的降解。This experiment was conducted to investigate the effect of the ratio of Leymus chinensis and Urtica cannbinauon rumen fermentation parameters in vitro.The experiment was divided into 5 groups according to the ratio of Leymus chinensis and Urtica cannbinau：0∶100（A）,30∶70（B）,50∶50（C）,70∶30（D）,100∶0（E）for the in vitro culture.The rumen fluid was collected to detect the fermentation parameters at 0,1,3,6,12 and 24 h.The results showed that：（1）pH was higher in group A and group C at 1,3,6 h（P〈0.05）,with the highest value falling in group C（P〈0.05）.（2）the groups D and E had a higher NH_3-N concentration at 3,6,12,24 h（P〈0.05）.（3）Microbial protein（MCP）was higher in group A and group C at 1,3,6 and 24 h（P〈0.05）,and the highest value was observed in group C（P〈0.05）.（4）Gas yield was higher in group A,group B and group C during the periods of 0 to 1 h,1 to 3 h（P〈0.05）,and it was the highest in group C among groups.（5）The digestibility of NDF was the highest in group C（P〈0.05）.In summary,Leymus chinensis and Urtica cannbinau ratio of 50∶50 in substrate showed comparatively higher pH and lower ammonia concentration.At the same time,the microbial protein content,gas production and 24 hNDF degradation rate were also higher.These results indicated Leymus chinensis to Urtica cannbinauratio of 50∶50 was beneficial to the fermentation of rumen microbes and the digestibility of substrate.

关 键 词：荨麻 羊草 体外发酵 瘤胃微生物 

分 类 号：S811.5[农业科学—畜牧学]