推拿联合跑轮训练对大鼠失神经骨骼肌萎缩的效果  

Effect of Tuina and Treadmill Training on Skeletal Muscle Atrophy after Denervation in Rats

作　　者：马颖[1] 严隽陶[1] 马书杰[2] 陆永嘉[2] 王春红[1] 史智君 陶然[2] 孔亚敏 MA Ying;YAN Jun-tao;MA Shu-jie;LU Yong-jia;WANG Chun-hon;SHI Zhi-jun;TAO Ran;KONG Ya-min(Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditonal Chinese Medicine, Shanghai 200437, China;Shanghai University of Traditional Chinese Medicine, Shanghai 201203, China)

机构地区：[1]上海中医药大学附属岳阳中西医结合医院推拿科,上海市200437 [2]上海中医药大学,上海市201203

出　　处：《中国康复理论与实践》2018年第5期530-534,共5页Chinese Journal of Rehabilitation Theory and Practice

基　　金：国家自然科学基金项目(No.30973806);国家自然基金青年科学基金项目(No.81603713);上海市中医药发展三年行动计划项目(No.ZY3-LCPT-1-1008)~~

摘　　要：目的探讨推拿手法联合跑轮训练对失神经骨骼肌萎缩的作用及其机制。方法 1月龄Sprague-Dawley大鼠80只,切断右下肢坐骨神经后随机分为对照组和手法组,每组40只。手法组予捏法推拿联合跑轮训练,对照组不予干预。分别在干预后1、2、3、4个月,每组各取10只大鼠检测腓肠肌湿重、肌卫星细胞计数,免疫组化检测胰岛素样生长因子Ⅰ(IGF-Ⅰ)阳性细胞计数,腓肠肌行HE染色观察。结果与对照组相比,手法组肌湿重比干预后3个月降低(F=4.590,P<0.05);肌卫星细胞计数在干预后3个月明显增加(F=12.466,P<0.01),IGF-Ⅰ阳性细胞数在干预后2个月后均增加(F>6.489,P<0.05)。HE染色示,手法组损伤程度有所减轻。结论推拿手法联合跑轮训练能够在一定程度上减轻失神经骨骼肌损伤,但不足以减轻肌萎缩,这可能是通过促进肌卫星细胞和IGF-Ⅰ的表达实现的。Objective To explore the effect of the combination of Tuina and treadmill training on denervation skeletal muscle atrophy.Methods A total of 80 Sprague-Dawley rats（one month old） were randomly divided into control group（n=40） and manipulation group（n=40）. Their sciatic nerves were transected, and the manipulative group accepted treadmill training and kneading of Tuina, while the control group accepted no intervention. Their muscle wet weight ratio,muscle satellite cells and insulin-like growth factor Ⅰ（IGF-Ⅰ） positive cells count were measured, and HE staining of gastrocnemius muscle were observed one, two, three and four months after intervention, ten rats in each group.Results Compared with the control group, the muscle wet weight ratio decreased three months after intervention（F=4.590, P〈0.05）, muscle satellite cells increased three months after intervention（F=12.466, P〈0.01）, and IGF-Ⅰ positive cells increased two, three and four months after intervention（F〉6.489, P〈0.05）. HE staining showed the skeletal muscle injury relieved somehow.Conclusion The combination of Tuina and treadmill training can relieve denervation skeletal muscle injury, but it is not enough for skeletal muscle atrophy, which may associate with promoting the expression of muscle satellite cells and IGF-Ⅰ.

关 键 词：骨骼肌萎缩 失神经 推拿 运动训练 肌卫星细胞 胰岛素样生长因子 大鼠 

分 类 号：R746.4[医药卫生—神经病学与精神病学]