PP242对人晶状体上皮细胞凋亡的诱导及凋亡相关蛋白Bax表达的影响  

Effect of PP242 on Apoptosis and Expression of the Apoptosis Protein Bax in Human Lens Epithelial Cells

作　　者：白雪[1] 宁宏[1] BAI Xue;NING Hong(Department of Ophthalmology, The First Hospital, China Medical University, Shenyang 110001, China)

机构地区：[1]中国医科大学附属第一医院眼科,沈阳110001

出　　处：《中国医科大学学报》2018年第6期548-551,共4页Journal of China Medical University

基　　金：辽宁省社会发展攻关计划(2013225303)

摘　　要：目的研究PP242对人晶状体上皮细胞(HLECs)凋亡的诱导及凋亡蛋白Bax表达的影响。方法用不同浓度PP242处理人晶状体上皮细胞系SRA01/04,分别用浓度为0、250、500、750、1 000 nmol/L的PP242处理HLECs 48 h后,Annexin V/PI双染流式细胞仪检测细胞凋亡率;采用实时定量PCR及Western blotting方法检测Bax的m RNA及蛋白的表达。结果 250、500、750和1 000 nmol/L PP242对人晶状体上皮细胞系SRA01/04作用48 h后,早期凋亡率分别为7.20%±0.36%、13.77%±0.21%、16.31%±0.20%和18.67%±0.49%,与对照组(0 nmol/L)差异有统计学意义(P<0.05)。Western blotting结果显示,随着PP242浓度的增加,Bax蛋白表达量增加,与对照组(0 nmol/L)比较差异有统计学意义(P<0.05);实时定量PCR结果显示,Bax m RNA表达量随PP242药物浓度的增加而增加,相对定量m RNA结果分别为2.157±0.125、3.733±0.302、5.596±0.261和7.436±0.167,与对照组(0 nmol/L)比较差异有统计学意义(P<0.05)。结论 PP242可以诱导HLECs的凋亡,增加其凋亡蛋白Bax的表达。Objective To study the effect of PP242 on apoptosis and the expression of the apoptosis protein Bax in human lens epi-thelial cells （HLECs） . Methods Immortal HLECs SRA01/04 were cultured and treated with different concentrations （0, 250, 500,750, or 1 000 nmol/L） of PP242. Forty-eight hours after PP242 treatment, the apoptotic rate of cells in each group was examined with flow cytometry （FCA, Annexin V ＋ PI staining） . Using reverse transcription quantitative polymerase chain reaction and Western blotting, the mRNA and protein levels of Bax were examined. Results Forty-eight hours after treatment with 250, 500, 750, or 1 000 nmol/L PP242,the apoptotic rate of HLECs was 7.20%± 0.36%, 13.77%± 0.21%, 16.31%± 0.20%, or 18.67%± 0.49%, respectively. Apoptotic rate in the control group was significantly different （P 〈 0.05） than that in the treatment groups. Results of the Western blotting revealed that the level of Bax was significantly increased. Further, the results of reverse-transcription quantitative PCR showed that expression of Bax mRNA increased in the treatment groups. The relative quantification of Bax mRNA was 2.157±0.125, 3.733±0.302, 5.596±0.261,7.436±0.167. Compared with the control group, the difference was statistically significant （P 〈 0.05） . Conclusion PP242 induces the expression of Bax and promotes apoptosis in cultured HLECs.

关 键 词：PP242 人晶状体上皮细胞 BAX 

分 类 号：R776.1[医药卫生—眼科]