结直肠癌患者维生素D受体对血浆促炎性细胞因子和肠道菌群的影响  

Effect of Vitamin D Receptor on Plasma Pro-inflammatory Cytokines Levels and Gut Microbiota in Patients with Colorectal Cancer

作　　者：于鑫 张永镇 于恩达[2] 王娜[1] 帅群 闫飞虎[2] 蒋露芳[1] 王和兴[1] 刘健翔 陈跃[4] 李兆申[2] 蔡全才[2] 姜庆五[1] YU Xin1,ZHANG Yong-zhen2,3,yu En-da2,et al.(1 .School of Public Health ,Fudan University/Key Laboratory of Public Health Safety of Ministry of Education,Shanghai 200032,China;2.Changhai Hospital of Second Military Medical University of Chinese PLA,Shanghai 200433,China;3.The 92914th Military Hospital of PLA,Lingao 571833,Chin)

机构地区：[1]复旦大学公共卫生学院/教育部公共卫生安全重点实验室,上海200032 [2]第二军医大学附属长海医院,上海200433 [3]中国人民解放军92914部队医院,海南临高571833 [4]加拿大渥太华大学,加拿大渥太华KIG523

出　　处：《中国肿瘤》2018年第5期382-386,共5页China Cancer

基　　金：国家自然科学基金项目(81473045,81273154)

摘　　要：[目的]研究结直肠癌患者维生素D受体（VDR）表达对血浆促炎性因子以及肠道菌群的影响情况。[方法]纳入初次诊断为结直肠癌的患者130例和年龄、性别成组匹配的对照（无结直肠疾病的受检者）130名,采用免疫组化的方法测量结直肠癌患者癌组织中的VDR表达水平,并将患者分为VDR高表达组以及VDR低表达组。检测研究对象血浆中促炎性细胞因子白介素-6（IL-6）、C反应蛋白（CRP）和肿瘤坏死因子受体2（s TNFRⅡ）水平,对研究对象粪便样本中细菌的16S r DNA进行测序得到肠道菌群构成与丰富度。[结果]VDR低表达组患者血浆s TNFRⅡ与CRP显著高于对照组（P〈0.001）。VDR低表达组s TNFRⅡ显著高于VDR高表达组（P=0.027）。VDR低表达组与高表达组分别与对照组相比得到的差异细菌不同,VDR低表达患者体内Fusobacterium varium、Ruminococcus torques等6种细菌有显著升高。[结论]结直肠癌患者体内VDR受体表达可能影响了血浆促炎性因子和肠道菌群变化。[Purpose] To investigate the effects of vitamin D receptor（VDR） on plasma pro-inflammatory cytokine levels and the gut microbiome in colorectal cancer（CRC） patients. [Methods] One hundred thirty CRC patients and 130 gender and age-matched healthy controls were included in the study. The expression of VDR protein in the tissue samples of 130 CRC patients was tested by immunohistochemical technique. The plasma levels of pro-inflammatory cytokines IL-6,C-reactive protein（CRP） and soluble tumor necrosis factor receptor Ⅱ（s TNFRⅡ） were examined. Composition and abundance of microbiota in stool were determined by 16 S r DNA sequencing. [Results] Among130 patients,there were 74 patients with low expression of VDR [VDR（-） group] and 56 patients with high expression of VDR[VDR（＋） group]. The plasma STNFRⅡ and CRP levels were significantly increased in VDR（-） group compared with control group（P〈0.001）. The level of s TNFRⅡ in VDR（-） group was significantly higher than that in VDR（＋） group（P =0.027）. The elevated abundance of Fusobacterium varium,Ruminococcus torques,and other four species was observed in VDR（-） group. [Conclusion] The expression of VDR in CRC patients may be associated with the changes in plasma pro-inflammatory cytokines and gut microbiota.

关 键 词：维生素D 维生素D受体 促炎性细胞因子 肠道菌群 结直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]