日光温室不同结果枝类型对柑橘果实有机酸含量的影响  

Effect of different types of fruiting shoots on organic acid content in citrus fruit grown in a solar greenhouse

作　　者：孙晓华[1] 叶丽红[1] 刘杰才[1] 李晓静[1] 张之为[1] 崔世茂[1] 宋阳[1] SUN Xiaohua, YE Lihong, LIU Jiecai, LI Xiaojing, ZHANG Zhiwei, CUI Shimao, SONG Yang(College of Agronomy, Inner Mongolia Agricultural University. Inner Mongolia Engineering Research Center of Facility Horticulture, Hohhot 010019, Inner Mongolia, Chin)

机构地区：[1]内蒙古农业大学农学院.内蒙古设施园艺工程技术研究中心,呼和浩特010019

出　　处：《果树学报》2018年第5期565-573,共9页Journal of Fruit Science

基　　金：内蒙古自治区自然科学基金(2015BS0317)

摘　　要：【目的】探明日光温室中柑橘结果枝类型对果实中有机酸含量的影响,为温室柑橘结果枝组的培养提供理论依据。【方法】以枳[Poncirus trifoliate(L.)Raf.]砧‘南丰蜜橘’(Citrus reticulate Blanco‘Kinokuni’)有叶结果枝和无叶结果枝果实为试材,对其糖酸含量、有机酸代谢相关酶活性以及基因表达量进行分析。【结果】‘南丰蜜橘’果实发育过程中蔗糖含量呈上升趋势,奎宁酸含量呈下降趋势,葡萄糖和果糖含量为先降后升,柠檬酸含量在果实成熟时与发育初期相近;无叶果和有叶果的糖酸含量变化趋势基本一致,但花后30 d无叶果苹果酸含量显著高于有叶果;2类果实中柠檬酸合成酶(citrate synthase,CS)、磷酸烯醇式丙酮酸羧化酶(phosphoenolpyruvate carboxylase,PEPC)、乌头酸酶(aconitate hydratase,ACO)和苹果酸脱氢酶(malate dehydrogenase,MDH)4种重要酶活性变化趋势基本一致;2类果实多数基因的表达量都存在显著性差异,然而酶活性以及有机酸含量则未出现相应的显著性变化。【结论】‘南丰蜜橘’果实中糖酸含量以及有机酸代谢相关重要酶活性不受结果枝组类型的影响,因此,在日光温室中进行柑橘树体枝组修剪时,只需考虑果实产量即可,不必过多考虑其对果实糖酸等内在品质的影响。【Objective】In order to provide a reference for pruning of fruiting branches in greenhousegrown citrus, the experiment was carried out to explore the influence of different types of fruiting branches on the content of organic acids in citrus fruit.【Methods】The experiment used‘Nanfeng tangerine＇（Citrus reticulate Blanco‘Kinokuni＇） grafted on the rootstock of trifoliate orange [Poncirus trifoliate（L.） Raf.] as material. Gas chromatography was used to measure the contents of sugars and organic acids. The activities of enzymes involved in tricarboxylic acid cycle（TCA） were analyzed, and key genes related to organic acid metabolism were analyzed by real-time quantitative reverse transcription polymerase chain reaction.【Results】Sucrose contents in fruits from both the leafy and leafless bearing shoots showed an increasing trend throughout the whole development period, and the accumulation of sucrose was accelerated from 120 days after flowering（DAF）. Quinic acid content presented a decreasing trend during fruit development in both shoot types, and the decrease amplitude was largest from 30 DAF to 90 DAF, after which quinic acid content became relatively constant as fruit matured.Glucose and fructose levels dropped sharply from 30 DAF, and began to rise slowly from 60 DAF. Thechange trends of glucose and fructose were similar with slight fluctuation during the whole development period. Sucrose content was much higher than those of glucose and fructose when the fruit ripened. During the whole development period, changes in citric acid content in fruit from the two types of bearing shoots were similar but relatively complicated with fluctuations. The differences in citric acid content between the two types fruiting shoots were not significant. The change in malic acid content in fruit from the leafy and leafless shoots was great from 30 DAF to 120 DAF, and leveled off from 120 DAF. The change pattern of malic acid in the two shoot types was similar, but malic acid content in fruit from t

关 键 词：'南丰蜜橘’ 有机酸 基因表达 结果枝 果实品质 

分 类 号：S666.2[农业科学—果树学]