毛白杨雄花序的化学成分研究  

Chemical constituents from the male anthotaxy of Populus tomentosa Carr.

作　　者：侯勇[1] 张广杰 崔慧敏 田瑛 刘士军 陈立 李彬 董俊兴[1,2] HOU Yong1, ZHANG Guang-jie2, CUI Hui-min2, TIAN Ying2, LIU Shi-jun2, CHEN Li2, LI Bin2, DONG Jun-xing1,2(1. Anhui Medical University, Hefei 230032, China; 2. Institute of Radiation Medicine, Academy of Military Medical Sciences, Academy of Military Sciences, Beijing 100850, Chin)

机构地区：[1]安徽医科大学,合肥230032 [2]军事科学院军事医学研究院放射与辐射医学研究所,北京100850

出　　处：《国际药学研究杂志》2017年第12期1131-1136,共6页Journal of International Pharmaceutical Research

摘　　要：目的探究中药杨树花的化学成分。方法采用ODS、硅胶、Sephadex LH-20柱色谱、重结晶等方法进行分离纯化,结合理化性质并利用ESI-MS、NMR等波谱学技术对分离所得化合物进行结构鉴定。采用ABTS快速法测定部分化合物抗氧化活性,MTT法检测部分化合物抗肿瘤活性。结果从毛白杨雄花序的70%乙醇提取物乙酸乙酯部位分离得到14个单体化合物,分别鉴定为乔松素(1),五桠果素(2),金圣草黄素(3),柚皮素(4),异野樱素(5),芹菜素(6),山柰酚(7),芹菜素-7-O-(-6″-p-羟基肉桂酰)-β-D-吡喃葡萄糖苷(8),水杨苷-2′-苯甲酸酯(9),邻苯二酚(10),3,3′,4,4′-四羟基联苯(11),香豆酸(12),熊果酸(13),桦木酮酸(14)。ABTS快速法结果显示,化合物2、6和7总抗氧化能力分别为0.61、0.14和0.46。体外抗肿瘤活性实验结果显示,化合物1、4和5对人非小细胞肺癌细胞A549的IC50值分别为26.04、43.45和24.01 mg/L;对人肝癌细胞Hep G2的IC50值为13.50、23.80和9.13 mg/L;对人结肠癌细胞HCT116的IC50值为36.11、48.95和32.25 mg/L。结论化合物2、3、11、13和14为首次从杨属植物中分离得到,化合物2~5、10、11和13~15为首次从该植物中分离得到。化合物2、6、7有一定的抗氧化能力;化合物1、4、5对肿瘤细胞有一定的抑制作用。Objective To study the chemical constituents of the male anthotaxy of Populus tomentosa Carr. Methods The compounds were isolated and purified by ODS,silica gel,Sephadex LH-20 column chromatography and recrystallization. Their structures were identified based on the physiochemical properties and spectroscopic data. The antioxidant capacities of some compounds were assayed by a rapid ABTS method. The antitumor activity was tested by the MTT assay. Results Fourteen compounds were isolated from the ethyl acetate part of 70% ethanol extract of the male anthotaxy of P. tomentosa Carr.,and they were identified as pinocembrin（1）,dillenetin（2）,chrysoeriol（3）,naringenin（4）,isosakuranetin（5）,apigenin（6）,kaempferol（7）,apigenin-7-O-β-D（-6″-pcoumaroyl）-glucoside（8）,tremuloidine（9）,catechol（10）,3,3′,4,4′-tetrahydroxybiphenyl（11）,coumaric acid（12）,ursolic acid（13）,and betulonic acid（14）,respectively. The results of the ABTS assay showed that 2,6 and 7 could scavenge ABTS＋free radicals with the total antioxidant capacity of 0.61,0.14,0.46 respectively. The screening results of antitumor activity in vitro showed that the half inhibitory concentration（IC50）of compounds 1,4 and 5 for adenocarcinomic human alveolar basal epithelial cells A549 was26.04 mg/L,43.45 mg/L and 24.01 mg/L,for human hepatocellular liver carcinoma cells Hep G2 were 13.50 μg/ml,23.80 μg/ml and9.13 μg/ml,and for human colon carcinoma cells HCT116 was 36.11 mg/L,48.95 mg/L and 32.25 mg/L.Conclusion Compounds2,3,11,13 and 14 were isolated from the Populus genus for the first time,2-5,10,11 and 13-15 were isolated from the plant for the first time. Compounds 2,6 and 7 displayed the antioxidant activity to a certain extent. Compouds 1,4 and 5 showed varying degrees of inhibitory effects on tumor cells in vitro.

关 键 词：毛白杨 化学成分 抗氧化能力 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学]