检索规则说明:AND代表“并且”;OR代表“或者”;NOT代表“不包含”;(注意必须大写,运算符两边需空一格)
检 索 范 例 :范例一: (K=图书馆学 OR K=情报学) AND A=范并思 范例二:J=计算机应用与软件 AND (U=C++ OR U=Basic) NOT M=Visual
作 者:于双妹 魏雪梅 张娜[1] 宋丽婷[1] 周海舟[1] Yu Shuangmei , Wei Xuemei, Zhang Na,Song Liting,Zhou Haizhou(Department of Laboratory Diagnosis,the First Affiliated Hospital of Harbin Medical University,Harbin 150001, Chin)
机构地区:[1]哈尔滨医科大学附属第一医院检验科,150001
出 处:《国际免疫学杂志》2018年第2期148-153,共6页International Journal of Immunology
基 金:国家自然科学基金(81772261)
摘 要:目的通过分析不同嗜性B亚型HIV-1感染者V3区氨基酸序列差异及感染实验,探讨V3区11、22和25位点与病毒感染能力及亲嗜性的关系。方法从HIV数据库中以FASTA格式下载B亚型V3区氨基酸序列,并将其分为CCR5亲嗜性组和CXCX4亲嗜性组,利用CLUSTAL W软件分别对两组序列进行多重序列比对,计算每个位点氨基酸出现的频率,并进行降幂排列。对HIV-1 HXB2和SF162株包膜糖蛋白V3区进行修饰,构建伪病毒及进行感染实验。结果研究结果显示,R5亲嗜性和X4亲嗜性病毒中V3区Consensus序列在11、22和25位上有差别。在R5亲嗜性毒株中这三个位点最常出现的氨基酸频率分别为71.9 % S、66.7 % A和56.0 % D;在X4亲嗜性毒株中这三个位点最常出现的氨基酸频率分别为50.0 % R、57.1 % T和26.2 % Q。V3区22氨基酸残基与病人CD4+T细胞数具有明显的相关性。R5亲嗜性或X4亲嗜性毒株V3区11、22和25位氨基酸残基的改变都使其感染能力降低,并且SF162的11位氨基酸残基S转变为R时,病毒由R5嗜性变为R5X4双嗜性。结论B亚型的HIV-1中V3区22位氨基酸残基与病毒的感染能力及病人的疾病进展具有明显的相关性,可以和11、25位氨基酸一起作为生物学表型预测的位点。ObjectiveOur goal in this study was to analyze position 11, 22 and 25 of the V3 loop associated with co-receptor usage and disease progression through comparing the amino acid sequences and infectivity of the V3 loop of HIV-1 subtype B infection.MethodHIV-1 subtype B V3 amino acid sequence were collected from the HIV seqquence database and divided into CCR5-tropic and CXCR4-tropic.The amino acid sequences of different tropism were multiple-aligned with CLUSTAL W program, and the frequencies of the amino acids sequences of two groups were calculated and sorted in descending order.We constructed pseudoviruses by changing the amino acids at position 22 of the V3 region in CCR5-tropic and CXCR4-tropic viruses and tested their infectivty.ResultThe amino acids at positions 11, 22, and 25 of V3 were different between the CCR5-tropic virus and CXCR4-tropic virus.The consensus amino acid frequencies were found to be 71.9% S, 66.7% A, and 56.0 % D for the CCR5-tropic virus and 50.0 % R, 57.1 % T, and 26.2 % Q for the CXCR4-tropic virus at positions 11, 22, and 25, respectively.There was a strong association between the identity of the residues at position 11, 22, and 25 of the V3 loop amino acid sequence and CD4+ T cell counts of different patients.All mutations at position 11, 22, 25 of the V3 region reduced its infectivity.When the position 11 of V3 loop of SF162 amino acid residues was converted to R, the virus tropism was changed from R5 to R5X4.ConclusionOur study indicates that position 22 of the V3 loop amino acid sequence is significantly associated with viral tropism and disease progression in HIV-1 subtype B.
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在载入数据...
正在链接到云南高校图书馆文献保障联盟下载...
云南高校图书馆联盟文献共享服务平台 版权所有©
您的IP:216.73.216.171