CREPT和CDK4在非小细胞肺癌组织中的表达及临床意义  

Expression and clinical significance of CREPT and CDK4 in non-small cell lung cancer

作　　者：夏靖华[1] 张志培[1] 文苗苗[1] 李维妙 孙盈[1] 王雪娇[1] 李小飞[1] XIA Jinghua;ZHANG Zhipei;WEN Miaomiao;LI Weimiao;SUN Ying;WANG Xuejiao;LI Xiaofei(Department of Thoracic Surgery,Tangdu Hospital,the Fourth Military Medical University, Xi？an 710038,China)

机构地区：[1]第四军医大学唐都医院胸腔外科,西安 710038

出　　处：《临床肿瘤学杂志》2018年第4期340-345,共6页Chinese Clinical Oncology

摘　　要：目的探讨CREPT与细胞周期蛋白依赖性激酶4(CDK4)在非小细胞肺癌(NSCLC)组织中的表达情况及临床意义。方法收集2006年3月至2011年12月手术切除的271例NSCLC组织及相应正常肺组织标本,采用免疫组化En Vision法检测上述组织中CREPT和CDK4的表达情况,分别采用RT-PCR和Western blotting检测NSCLC组织及相应正常肺组织标本中CREPT和CDK4的mRNA和蛋白表达。结果 CREPT与CDK4主要表达于细胞核。CREPT在NSCLC组织中的阳性表达率为96.7%(262/271),高于正常肺组织的42.1%(114/271),差异有统计学意义(P=0.000);CDK4在NSCLC组织中的阳性表达率为91.9%(249/271),高于正常肺组织的15.5%(42/271),差异有统计学意义(P=0.000)。CREPT表达与分化程度、TNM分期和淋巴结转移有关(P<0.05),CDK4表达与肿瘤直径、分化程度、TNM分期和淋巴结转移有关(P<0.05)。在NSCLC总体、腺癌、鳞癌组织中,CREPT和CDK4表达均呈正相关(r值分别为0.520、0.544、0.501,P=0.000)。Western blotting检测显示,CREPT和CDK4蛋白在NSCLC组织中表达,在相应正常肺组织中不表达。RT-PCR检测显示,CREPT和CDK4的mRNA水平在NSCLC组织中(0.2846±0.0780、0.4013±0.0677)明显高于正常肺组织(0.0894±0.0292、0.1882±0.0761),差异有统计学意义(P=0.024,P<0.001)。结论 CREPT和CDK4是NSCLC组织与相应正常肺组织表达的差异蛋白,二者表达均与NSCLC分化程度、TNM分期、淋巴结转移有关,在NSCLC发生、发展中起促进作用。Objective To investigate the expression and clinical significance of CREPT and cyclin-dependent kinase 4（ CDK4） in non-small cell lung cancer（ NSCLC） tissues. Methods We collected 271 cases of surgically resected NSCLC tissues and corresponding normal tissues from March 2006 to December 2011. The expression of CREPT and CDK4 of above tissues were detected by immunohistochemical En Vision mothod. The mRNA and protein levels of CREPT and CDK4 in NSCLC and correspongding normal tissues were detected by RT-PCR and Western blotting,respectively. Results Positive staining of CREPT and CDK4 were mainly in nucleus. The positive rate of CREPT was 96. 7%（ 262/271） in NSCLC tissues,higher than 42. 1%（ 114/271） of corresponding normal tissues（ P = 0. 000）. The positive rate of CDK4 was 91. 9%（ 249/271） in NSCLC tissues,higher than 15. 5%（ 42/271） of corresponding normal tissues（ P = 0. 000）. The expression of CREPT was correlated with differentiation,TNM staging and lymph node metastasis（ P〈0. 05）,and CDK4 was associated with tumor diameter,differentiation,TNM staging and lymph node metastasis（ P〈0. 05）. The expression of CREPT was positively correlated with CDK4 in NSCLC,adenocarcinoma,squamous cell carcinoma tissues（ r = 0. 520,0. 544,0. 501,P all = 0. 000）. Western blotting showed that the expression of CREPT and CDK4 could be found in NSCLC tissues,while they could not be found in corresponding normal tissues. The mRNA levels of CREPT and CDK4 in NSCLC tissues（ 0. 2846 ±0. 0780,0. 4013±0. 0677） were much higher than those in corresponding normal tissues（ 0. 0894 ± 0. 0292,0. 1882 ± 0. 0761） with statistic significance（ P = 0. 024,P〈0. 001）. Conclusion CREPT and CDK4 are differentially expressed proteins in NSCLC and corresponding normal tissues and they are closely related to differentiation degree,TNM staging and lymph node metastasis in NSCLC.CREPT and CDK4 promote the occurrence and development of NSCLC.

关 键 词：非小细胞肺癌(NSCLC) CREPT 细胞周期蛋白依赖性激酶4(CDK4) 

分 类 号：R734.2[医药卫生—肿瘤]