钙蛋白酶-9与胃癌发生及其转移的相关性研究  

Study on the relationship of the expression level of Calpain-9 with occurrence and metastasis of gastric carcinoma

作　　者：吴燕敏[1] 林刚[1] 余利华[1] 陆培华[2] 王文远[1] Wu Yanmin;LinGang;Yu Lihua;Lu Peihua;Wang Wenyuan(Department of Gastroenterology, the Third People＇s Hospital of Wuxi, Wuxi 214041, China（Wu YM, Lin G, Yu LH, Wang WY;Department of Oncology, the People＇s Hospital of Wuxi, Wuxi 214043, China（ Lu PH)

机构地区：[1]无锡市第三人民医院消化内科,214041 [2]无锡市人民医院肿瘤科,214043

出　　处：《中华老年医学杂志》2018年第5期522-524,共3页Chinese Journal of Geriatrics

基　　金：国家自然科学基金项目（81472305）

摘　　要：目的探讨胃癌组织中钙蛋白酶9表达与胃癌临床病理特征及转移的关系。方法采用免疫印记和免疫组织化学方法，检测钙蛋白酶-9在146例胃癌患者的癌组织及配对癌旁正常组织标本中的表达水平，探讨其与胃癌患者不同临床病理指标之间的关系。结果胃癌组织较配对癌旁正常胃黏膜组织钙蛋白酶-9表达水平显著降低，其表达水平在胃癌组织学分化类型、临床分期、淋巴转移之间差异有统计学意义（均P〈0．05）；而在胃癌患者性别、年龄、肿瘤大小及远处转移之间差异无统计学意义（均P〉0．05）。相关分析显示，胃癌组织中钙蛋白酶-9表达水平与淋巴转移呈负相关（r=-0．3826，P〈0．05）。结论钙蛋白酶-9在人胃癌组织中表达降低，其表达水平与胃癌患者的淋巴结转移呈负相关。Objectives To explore the correlation of the expression level of Calpain-9 in gastric carcinoma with clinico-pathological features and metastasis of gastric cancer. Methods The expression level of Calpain-9 in gastric carcinoma and matched para-cancerous normal mucosa tissues were detected by Western blotting and immunohistochemistry in 146 patients with gastric cancer respectively. The relationship between the expression level of Calpain-9 and the various clinicopathological indexes was analyzed. Results The expression level of Calpain-9 was significantly lower in cancerous tissues than in matched para cancerous normal mucosa tissues （P 〈 0.05）, with significantly different expression levels of Calpain-9 in different gastric cancerous histological differentiation degrees,different clinical stages, and with or without a lymph node metastasis（all P〈 0.05）. But no statistical difference was found in different sexes,different ages,different tumor sizes, with or without a distant metastasis（all P〉0.05）. The spearman＇s correlation analysis showed that the expression level of Calpain-9 in gastric cancer was negatively correlated with lymph node metastasis （r=-0. 3826, P〈0.05）. Conclusions The expression level of Calpain-9 in gastric cancer tissues is decreased,is negatively correlated with clinical stages, pathological differentiation, and cancerous lymph node metastasis.

关 键 词：胃癌 肿瘤转移 钙蛋白酶-9 

分 类 号：R735.2[医药卫生—肿瘤]