基于气相色谱-质谱联用代谢组学方法的甘肃不同产地当归中挥发性成分与其生长环境关系研究  

Relationship Between Volatile Components in Angelicae Sinensis Radix from Different Regions in Gansu Province and Its Growing Environment with GC-MS-based Metabolomics

作　　者：萨日娜[1,2] 潘新波[3] 顾志荣[1,2] 刘洁丽[1] 孙宇靖 王亚丽[1,4] SA Ri-na;PAN Xin-bo;GU Zhi-rong;LIU Jie-li;SUN Yu-jing;WANG Ya-li(Gansu University of Chinese Medicine, Lanzhou 730000, China;Gansu Provincial Hospital, Lanzhou 730000, China;The Second People’s Hospital of Lanzhou, Lanzhou 730000, China;Key Laboratory of TCM Quality and Standard of Gansu Province, Lanzhou 730000, China)

机构地区：[1]甘肃中医药大学,甘肃兰州730000 [2]甘肃省人民医院,甘肃兰州730000 [3]兰州市第二人民医院,甘肃兰州730000 [4]甘肃省中药质量与标准研究重点实验室,甘肃兰州730000

出　　处：《中国中医药信息杂志》2018年第6期82-86,共5页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：国家自然科学基金(30960037)

摘　　要：目的基于气相色谱-质谱联用(GC-MS)代谢组学方法探讨甘肃不同产地当归中挥发性成分与药材生长环境的关系。方法采用GC-MS技术测定31批甘肃不同产地当归药材样品中挥发性成分,并通过无监督模式的主成分分析(PCA)和有监督模式的偏最小二乘分析(PLS)对甘肃不同产地当归药材与其生长环境的关系进行分析和评价。结果 PCA结果表明,甘肃不同产地当归中挥发性成分与海拔、土壤类型具有相关性;PLS可将31批甘肃不同产地的当归样品依据海拔差异明显分为3类,不同海拔的当归样品中挥发性成分存在较为明显的差异;分析对比分析PCA和PLS得到的线性载荷图,可识别筛选出11个差异特征性成分,通过NIST11数据库检索,鉴定出其中7个差异性化合物。结论甘肃不同产地当归中挥发性成分与其生长的海拔、土壤类型的交互影响具有明显相关性。Objective To explore the relationship between the volatile components in Angelicae Sinensis Radix from different regions of Gansu Province and its growing environment with metabolomics based on GC-MS. Methods The GC-MS method was used for detecting the volatile components in Angelicae Sinensis Radix from 31 different regions in Gansu province, and principal component analysis（PCA） and partial least squares（PLS） methods were used for analyzing and evaluating its relationship with the growing environment. Results The results of PCA showed that the volatile components in Angelicae Sinensis Radix from different regions in Gansu province were related to the altitude and the soil types. The PLS method could divide 31 samples of Angelicae Sinensis Radix from different regions in Gansu Province into three groups according to the difference of altitude. There were significant differences in the volatile components in the samples taken at different altitude regions. After analyzing linear loading plots from PCA and PLS, 11 charateristic components were screened out, including 7 compounds were identified by the retrieval of NIST11 database. Conclusion The volatile components in Angelicae Sinensis Radix from different regions in Gansu Province are closely related to the altitude and the soil type.

关 键 词：当归 甘肃 不同产地 气相色谱-质谱联用 主成分分析 偏最小二乘法 

分 类 号：R284.1[医药卫生—中药学]