不同配伍组方对麻黄细辛附子汤体外经皮渗透行为的影响  

Influence of Different Compatibility Prescriptions on in Vitro Percutaneous Penetration of Mahuang Xixin Fuzitang

作　　者：王艳宏[1] 赵雪[1] 刘书博 关枫[1] 李伟男[1] 李永吉[1] 陈大忠 WANG Yan-hong;ZHAO Xue;LIU Shu-bo;GUAN Feng;LI Wei-nan;LI Yong-ji;CHEN Da-zhong(Research Institute of Chinese Medicine, College of Traditional Chinese Medicine, Heilongjiang University of Chinese Medicine, Harbin 150040, China)

机构地区：[1]黑龙江中医药大学药学院中医药研究院

出　　处：《中国实验方剂学杂志》2018年第11期1-8,共8页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81473359);黑龙江省科技厅青年基金项目(QC07C107);黑龙江省教育厅科学技术研究项目(11531357)

摘　　要：目的：探讨不同配伍组方对麻黄细辛附子汤（MXF）体外经皮吸收的影响,以阐明MXF外用的配伍机制,为其经皮给药提供科学依据,也为中药复方外用配伍机制研究积累数据。方法：采用Franz体外扩散池法,以含1%聚山梨酯-80的生理盐水溶液为接受液,采用麻黄生物碱类成分（盐酸麻黄碱和盐酸伪麻黄碱）,附子生物碱类成分（苯甲酰新乌头原碱、苯甲酰次乌头原碱和苯甲酰乌头原碱）和细辛挥发油类成分（细辛脂素和甲基丁香酚）的12 h累积渗透量（Q12 h）和稳态渗透速率（Jss）为指标,考察麻黄组、附子组、细辛组、麻黄-附子组、麻黄-细辛组、附子-细辛组、麻黄-附子-细辛组（全方组）对大鼠背部的透皮吸收情况。结果：各成分的Q12 h和Jss比较,盐酸麻黄碱、盐酸伪麻黄碱排序均为全方组〉麻黄-细辛组〉麻黄组〉麻黄-附子组,且盐酸麻黄碱〉盐酸伪麻黄碱;苯甲酰新乌头原碱、苯甲酰次乌头原碱和苯甲酰乌头原碱排序均为附子-细辛组〉附子组〉麻黄-附子组〉全方组,且苯甲酰新乌头原碱〉苯甲酰乌头原碱〉苯甲酰次乌头原碱;甲基丁香酚排序为细辛组〉麻黄-细辛组〉附子-细辛组〉全方组。各组中细辛脂素的Q12 h和Jss差异不大。结论：不同配伍组方对MXF中各指标成分的经皮吸收有影响,且对不同成分的影响程度不同;成分不同,经皮吸收效果也不同。细辛对盐酸麻黄碱、盐酸伪麻黄碱、苯甲酰新乌头原碱、苯甲酰次乌头原碱和苯甲酰乌头原碱的经皮吸收具有一定的促进作用,附子对盐酸麻黄碱和盐酸伪麻黄碱的经皮吸收均有一定抑制作用,麻黄和附子均会对甲基丁香酚的经皮吸收起到显著抑制作用。Objective： To elucidate the compatibility mechanism of Mahuang Xixin Fuzitang(MXF） for external use and accumulate data for the study of compatibility mechanism of Chinese herbal prescription for external use,the influence on in vitro skin permeation profiles of MXF composed by different prescription was investigated in this paper. Method： The rat back percutaneous permeation studies of Mahuang group,Fuzi group,Xixin group,Mahuang Fuzi group,Mahuang Xixin group,Fuzi Xixin group,Mahuang Fuzi Xixin group(the whole prescription group） were carried out to compare the permeation effect of different prescriptions by in vitro Franz diffusion cell method, taking normal saline solution containing 1% polysorbate-80(tween-80） as the receiving solution,cumulative permeation quantity in 12 h(Q12 h） and steady penetration rate(Jss） of ephedrine hydrochloride, pseudoephedrine hydrochloride, benzoylmesaconine, benzoylhypaconine, benzoylaconine,methyleugenol and asarinin as indexes. Result： Comparison of Q12 hand Jssof various components,the rank order of ephedrine hydrochloride or pseudoephedrine hydrochloride was whole prescription group 〉Mahuang Xixin group Mahuang group〉 Mahuang Fuzi group, and ephedrine hydrochloride 〉pseudoephedrine hydrochloride;benzoylmesaconine,benzoylhypaconine and benzoylaconine were in the order of Fuzi Xixin group 〉Fuzi group 〉Mahuang Fuzi group 〉whole prescription group,and benzoylmesaconine 〉benzoylaconine 〉benzoylhypaconine;methyleugenol was ranked as Xixin group 〉Mahuang Xixin group〉 Fuzi Xixin group 〉whole prescription group;the difference of Q12 hand Jssof asarinin among groups was small. Conclusion： The percutaneous permeation of all kinds of components in MXF can be influenced by different compatibility,the impact was different according to different component. Asari Radix et Rhizoma can promote the percutaneous permeation of ephedrine hydrochloride,pseudoephedrine hydrochloride, benzoylmesaconine, benzoylhypaconine and benzoylaconine; Aconiti Lateralis

关 键 词：麻黄细辛附子汤 温经散寒 配伍 经皮渗透特性 拆方 生物碱 挥发油 

分 类 号：R22[医药卫生—中医基础理论] R24[医药卫生—中医学]