氢气干预抑制非小细胞肺癌生长的作用及其机制研究  

Effect and mechanism of hydrogen suppressing non-small cell lung cancer

作　　者：王东昌[1] 赵云霞[1] 赵志芳[1] 李亚华[1] 王丽飞[1] 陈刚[1] Wang Dongchang, Zhao Yunxia, Zhao Zhifang, Li Yahua, Wang Lifei, Chen Gang.(The First Department of Respiratory Medicine, the Third Hospital of Hebei Medical University, Shijiazhuang 050051, Chin)

机构地区：[1]河北医科大学第三医院呼吸一科,石家庄050051

出　　处：《国际呼吸杂志》2018年第8期561-565,共5页International Journal of Respiration

基　　金：2016年河北省卫计委青年科技课题（20160602）

摘　　要：目的 探讨氢气对非小细胞肺癌的抑制作用及其机制.方法 将16只BALB/c雄性荷瘤裸鼠随机分为对照组和氢气组,氢气组给予吸入60% 氢气＋40% 空气2 h/d处理.干预4周后结束实验,测量瘤体的体积及重量,采用苏木精-伊红染色法、免疫组织化学方法、实时定量聚合酶链式反应及Western blot检测肿瘤组织中Ki-67、环氧化酶2（COX-2）、血管内皮生长因子（VEGF）的含量.结果 与对照组相比,氢气组瘤体的体积及重量显著减小,氢气组 Ki-67、COX-2、VEGF的mRNA、蛋白表达显著减少,且其病理形态及细胞异质性相对于对照组存在显著差异.结论 氢气干预可抑制非小细胞肺癌生长,其机制与抑制Ki-67、COX-2、VEGF表达有关.Objective To investigate the inhibitory effect of hydrogen on non-small cell lung cancer and its mechanism.Methods Sixteen BALB/c male tumor-bearing nude mice were randomly divided into control group and hydrogen group.Hydrogen group was treated for four weeks （60% hydrogen＋ 40%air,two hours a day.The volume and weight of the tumor were measured.The levels of Ki-67, cyclooxygenase-2 （COX-2）,and vascular endothelial growth factor （VEGF） in the tumor tissues were detected by hematoxylin-eosin staining,immunohistochemistry,real-time quantitative polymerase chain reaction and Western blot.Results The tumor volume and weight of the hydrogen group were significantly lower than those of the control group.The mRNA and protein contents of Ki-67,COX-2 and VEGF in the hydrogen group were significantly reduced,and its pathological morphology and cellular heterogeneity were significantly different from the control group.Conclusions Hydrogen can inhibit non-small cell lung cancer,which is related to the repression of Ki-67,COX-2 and VEGF.

关 键 词：氢气 血管内皮生长因子 环氧化酶2 肺肿瘤 

分 类 号：R734.2[医药卫生—肿瘤]