透明托盘法使用生物活性玻璃对牙髓腔渗透性的影响  

Impact of tray-based bioactive glasses application on pulp permeability

作　　者：马骞[1] 乔萍丽[1] 陈亚明[1] MA Qian, QIAO Pingli, CHEN Yarning.(Jiangsu Key Laboratory of Oral Diseases, Nanjing Medical University ; Department of Polyclinics, Affiliated Hospital of Stomatology, Nanjing Medical University, Nanjing 210029, Chin)

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室南京医科大学附属口腔医院综合科,江苏南京210029

出　　处：《口腔医学》2018年第5期428-432,共5页Stomatology

基　　金：南京市科技计划项目(201503062);江苏高校优势学科建设工程资助项目(2014-037);南京医科大学口腔医学院横向合作项目(22011001)

摘　　要：目的将纳米级生物活性玻璃脱敏剂用于漂白治疗前的离体牙,观察比较未使用脱敏剂和分别用涂抹法和透明托盘法使用生物活性玻璃脱敏剂后牙髓腔内过氧化氢渗透量的多少。方法 48颗离体第一前磨牙分成6组,A组不做脱敏处理,牙冠倒置,冠部分浸泡于去离子水中30 min,B组表面不做脱敏处理,牙冠倒置于30%过氧化氢溶液中30 min,C组生物活性玻璃涂抹2 min,2次/d,浸没于人工唾液24 h后,牙冠倒置于30%过氧化氢溶液中,D组使用透明托盘+生物活性玻璃1 h,浸没于人工唾液24 h后,牙冠倒置于30%过氧化氢溶液30 min,E组生物活性玻璃涂抹2 min,2次/d,浸没于人工唾液24 h后,牙冠倒置于去离子水中30 min,F组使用透明托盘+生物活性玻璃1 h,浸没于人工唾液24 h后,牙冠倒置浸泡于去离子水中30 min,提取各组髓腔内液体,检测各组的吸光度,并换算成过氧化氢的总量。结果 6组髓腔内液过氧化氢含量分别为A组0μg,B组21.149μg±0.489μg,C组9.813μg±0.426μg,D组4.065μg±0.268μg,E组0μg,F组0μg,单因素方差分析表明,B、C、D组髓腔内的过氧化氢含量有差异,差异有统计学意义(F=459.748,P<0.05),B组>C组>D组。结论生物活性玻璃脱敏剂结合透明托盘的使用可以有效减少过氧化氢渗透进牙髓腔的量,降低过氧化氢对牙髓组织产生的有害影响。Objective Prior to the use of nano-BGs desensitizers in teeth bleaching operation,to observe the content of H2O2 in dentine pulp with or without the application of desensitizers by smearing method or transparent tray. Methods 48 dentin disks were collected from human first molars and divided into 6 groups. Group A： without desensitizing treatment,the crown ends of the disks were soaked into deionized water for 30 minutes. Group B： without desensitizing treatment,the crown ends of the disks were soaked into 30%H2O2; Group C： smearing application of BGs desensitizers for 2 min,twice/day,after soaking disks into artificial saliva（ AS） for 24 hours,the crown ends of the disks were put into 30% H2O2 for 30 minutes; Group D： transparent tray application of BGs desensitizers 1 hour,after soaking disks into artificial saliva for 24 hours,the crown ends of the disks were put into 30% H2O2 for 30 minutes; Group E： smearing application of BGs desensitizers for 2 min,twice/day,after soaking disks into artificial saliva for 24 hours,the crown ends of the disk were put into deionized water for 30 minutes; Group F： transparent tray application of BGs desensitizers for 1 hour,after soaking disks into artificial saliva for 24 hours,the crown ends of the disk were put into deionized water for 30 minutes. The fluid in dentin pulp of each group was extracted and the content of H2O2 was calculated by the measurement of absorbance with UV-visspectrophotometry. Results The contents of H2O2 in each group were： 0 μg（ A group）,21.149 μg± 0.489 μg（ B group）,9.813 μg ±0.426μg（ C group）,4.065 μg ±0.268 μg（ D group）,0 μg（ E group）,0 μg（ F group）. ANOVA results indicated that statistically significant difference existed between B,C and D（ F = 459.748,P〈0.05）. Multiple comparison results indicated that significant difference also existed between B,C and D. Conclusion The application of BGs desensitizers can reduce the penetration of H2O2 into dentin pulp so as to reduce the occurrence

关 键 词：生物活性玻璃 透明托盘 再矿化 漂白剂 髓腔渗透性 

分 类 号：R783.1[医药卫生—口腔医学]