模拟干旱胁迫下马铃薯StNCED1表达量及与ABA含量的相关性分析  

Correlation Analysis of StNCED1 Expression Level and ABA Content of Potato Under Simulated Drought Stress

作　　者：巩檑[1] 宋继玲[2] 甘晓燕[1] 刘璇 陈虞超[1] 郭志乾 宋玉霞[1] GONG Lei;SONG Ji-ling;GAN. Xiao-yanl;LIU Xuan;CHEN Yu-chao;GUO Zhi-qian;SONG Yu-xia(Agricultural Bio- Technology Centre, Ningxia Academy of Agriculture and Forestry Science, Yinchuan 750002;Keshan Branch of Heilongjiang Academy of Agricultural Science ,Keshan 161600;Guyuan Institute of Agricultural Sciences, Ningxia Academy of Agriculture and Forestry Science, Guyuan 756000)

机构地区：[1]宁夏农林科学院农业生物技术研究中心,银川750002 [2]黑龙江省农业科学院克山分院,克山161600 [3]宁夏农林科学院固原分院,固原756000

出　　处：《植物遗传资源学报》2018年第3期561-567,共7页Journal of Plant Genetic Resources

基　　金：宁夏自治区农业育种专项(2014NYYZ01);宁夏农林科学院一二三产业融合发展科技创新示范项目(YES-16-0102);宁夏农林科学院科技创新先导资金项目(NKYG-15-06;NKYJ-17-03;NKYJ-17-07);宁夏自然科学基金项目(NZ16123;NZ16122;NZ17124)

摘　　要：干旱缺水是限制马铃薯产量和品质的关键因素之一。ABA是干旱胁迫应答基因调控网络中的重要组成;9-顺式-环氧类胡萝卜素双加氧酶(NCED)是植物ABA生物合成途径的关键限速酶,该基因的表达模式直接影响ABA代谢。但干旱胁迫下有关马铃薯NCED基因表达与激素、表型间的相关分析的研究尚少。本研究克隆了马铃薯NCED1基因的全长c DNA序列,检测了4个马铃薯种质材料在不同模拟干旱(PEG-6000)胁迫强度处理下NCED1基因表达量、ABA含量及根系长度间的相关性。结果表明,St NCED1全长2181 bp,包含一个1800 bp的完整开放读码框,编码599个氨基酸。5%PEG-6000和15%PEG-6000模拟干旱胁迫4周后,4个品种均表现出随胁迫强度的加重植株生长缓慢、矮小,根系长度显著降低。干旱敏感型材料早大白中根系长度变化最大,ABA含量显著高于其他3个种质材料。CIP478.9、star和米拉3份种质材料中St NCED1表达量与对照植株有显著差异,且随胁迫强度增加而增大;早大白中St NCED1表达量表现出先降低后升高趋势。模拟干旱胁迫下,ABA含量与St NCED1表达量之间呈现出正相关关系(R>0.7)。研究结果为解析马铃薯响应干旱胁迫的调节机制及其在抗旱种质资源筛选中的应用提供了基础数据。Drought is one of the most critical factor limiting the potato yield and quality. Abscisic acid（ABA） is the essential part of drought responsing regulatory networks and 9-cis-epoxycarotenoiddioxygenase （NCED） is the keyrate-limiting enzyme of ABA biosynthesis in higher plants. It directly influences the expression pattern of ABA metabolism. However, the relationship among NCED expression, ABA content and drought tolerance in potato re- mains unclear. Here, we isolated by sequence homology a NCED gene StNCED1 from potato and determined its gene expression, ABA content and root length in 4 varieties under simulated drought stress by different concentration of PEG- 6000. The full length of StNCED! was 2181 bp, containing a 1800 bp open reading frame （ORF） that encodes a deduced protein of 599 amino acid residues. Under treatments, the shoot growth and root length at the seedling stage of all the four varieties was significantly inhibited. Drought-sensitive material ＇ zaodabai ＇ showed the most obvious changes in root length among the 4 tested materials and the ABA content is significantly higher than the oth- er three materials. The expression level of StNCED1 under drought stress treatment was significantly higher than the control group of CIP478.9, star and mila,respectively. The expression levels were increased with increasing concen- tration of PEG-6000 in these three materials. Oppositely,StNCED1 expression and ABA content decreased at the early time-point and then increased in ＇ zaodabai＇. A positive correlation （ R 〉 0.7 ） was proved between ABA con- centration change and StNCED1 expression level in all the 4 tested materials under drought stress. Our results will provide fundermental data for further revealing the regulation mechanism of StNCED1 responsing to drought stress and will be helpful in screening drought resistance germplasm resources.

关 键 词：马铃薯 模拟干旱 ABA StNCED1表达 

分 类 号：S423[农业科学—植物保护] S532