煤制甲醇合成作业人员氧化损伤与hOGG1 mRNA表达的研究  

The Research of Oxidative Damage and Expression of hOGG1 mRNA in Coal Chemical Synthesis Workers

作　　者：李宣珠 陈楠 赵燕 刘贺荣 LI Xuanzhu;CHEN Nan;ZHAO Yan;LIU Herong(School of Public Health and Management,Ningxia Medical University,Yinchuan 75000)

机构地区：[1]宁夏医科大学公共卫生与管理学院,银川750004

出　　处：《宁夏医科大学学报》2018年第2期129-132,共4页Journal of Ningxia Medical University

基　　金：宁夏卫计委重点项目(2014-NW-002)

摘　　要：目的本研究通过调查某煤制甲醇厂煤制甲醇合成作业人员的氧化应激水平,研究煤制甲醇作业甲醇低浓度接触后氧化损伤hOGG1 mRNA表达的关系。方法从煤制甲醇厂选取甲醇合成工93名和管理人员86名为暴露组和对照组,测定两组人员血清中超氧化物歧化酶(SOD)活力、丙二醛(MDA)含量、谷胱甘肽过氧化物酶(GSH-PX)含量及血液hOGG1 mRNA表达,并对工人接触区进行现场空气采样及检测。结果作业场所空气检测甲醇、苯及苯系物、一氧化碳等职业性有害因素短时间接触浓度结果均未超过职业接触限值。SOD活性暴露组低于对照组,MDA含量暴露组高于对照组(P<0.01)。GSH-PK和hOGG1 mRNA表达水平两组间差异无统计学意义(P>0.05)。结论煤制甲醇工业为新型的化工行业,其对作业工人可造成氧化损伤,但h OGG1基因在煤制甲醇合成工的DNA氧化损伤修复能力效果不明显。Objective To investigate the oxidative stress level of workers exposed to methanol for a long time in the coal chemical industry and to study the relationship between oxidative damage and hOGG1 mRNA expression in low concentration methanol exposed to coal chemical industry. Methods A total of 93 methanol synthesis workers in the coal methanol plant were selected as the exposure group and 86 staff in the management department of the same plant as the control group. The activities or contents of MDA,SOD and GSH-PK and the expression of hOGG1 mRNA were measured. On-site air sampling and testing were made in contact area of workers. Results In workplace air detection,short exposure time results were lower than the exposure limit. Compared the differences of MDA,SOD,GSH-PK and expression of hOGG1 repair enzyme gene between exposed group and control group,MDA and SOD were statistically significant（P〈0.01）. The expressions of GSH-PK and mRNA showed no statistically difference（P〉0.05）. Conclusion Coal production of methanol industry is the new chemical industry. Coal production of methanol industry can cause oxidative damage to workers,hOGG1 gene in the coal methanol synthesis of DNA oxidative damage repair effect is not obvious.

关 键 词：氧化损伤 8-羟基鸟嘌呤糖苷酶 煤化工作业人员 

分 类 号：R363[医药卫生—病理学]