食管鳞癌患者血清差异表达miRNAs的筛选  

A preliminary screening of differentially expressed miRNAs in serum of patients with esophageal squamous cell carcinoma

作　　者：丁元杰 马箐 郭旭东 沈艺 魏文强 刘芬 DING Yuanjie;MA Qing;GUO Xudong;SHEN Yi;WEI Wenqiang;LIU Fen(School of Public Health,Capital Medical University,Beijing 100069,china)

机构地区：[1]首都医科大学公共卫生学院/北京市临床流行病学重点实验室,北京100069 [2]国家癌症中心、中国医学科学院北京协和医学院肿瘤医院

出　　处：《山东医药》2018年第15期1-4,共4页Shandong Medical Journal

基　　金：国家自然科学基金资助项目(81473056,81573224);北京市自然科学基金面上项目(7172025,7132023);北京市教委科研计划一般项目(SQKM201710025006)

摘　　要：目的初步筛选食管癌高发区食管鳞癌患者血清中差异表达的小分子RNAs(miRNAs)。方法选择52例食管鳞癌患者(观察组)、52名健康查体者(对照组),均抽取空腹静脉血4 m L。采用AgilentTM高通量miRNA微阵列芯片检测二者血清中miRNAs。结果与对照组比较,观察组26个miRNAs(hsa-let-7b-5p、hsa-miR-107、hsamiR-1207-5p、hsa-miR-1238-3p、hsa-miR-1246、hsa-miR-1290、hsa-miR-15a-5p、hsa-miR-16-5p、hsa-miR-19b-3p、hsamiR-223-3p、hsa-miR-25-3p、hsa-miR-320c、hsa-miR-3663-3p、hsa-miR-4281、hsa-miR-4455、hsa-miR-4516、hsa-miR-451a、hsa-miR-4532、hsa-miR-4634、hsa-miR-4687-3p、hsa-miR-4763-3p、hsa-miR-486-5p、hsa-miR-574-5p、hsa-miR-6090、hsa-miR-6515-3p、hsa-miR-92a-3p)的荧光信号强度FC绝对值均>2,P均<0.05,FDR均<0.1。结论食管鳞癌患者血清中hsa-let-7b-5p、hsa-miR-107、hsa-miR-1207-5p、hsa-miR-1238-3p、hsa-miR-1246、hsa-miR-1290、hsamiR-15a-5p、hsa-miR-16-5p、hsa-miR-19b-3p、hsa-miR-223-3p、hsa-miR-25-3p、hsa-miR-320c、hsa-miR-3663-3p、hsa-miR-4281、hsa-miR-4455、hsa-miR-4516、hsa-miR-451a、hsa-miR-4532、hsa-miR-4634、hsa-miR-4687-3p、hsa-miR-4763-3p、hsa-miR-486-5p、hsa-miR-574-5p、hsa-miR-6090、hsa-miR-6515-3p、hsa-miR-92a-3p差异表达。26个miRNAs可能与食管鳞癌的发生、发展有关。Objective To screen out the differentially expressed microRNAs（miRNAs） in the serum of esophageal squamous cell carcinoma（ESCC） patients from a high risk area. Methods The blood samples（4 m L per subject） from52 patients with newly diagnosed ESCC（observation group） and 52 age/sex-matched healthy controls（control group） were obtained from Linzhou,Henan province. The miRNAs were detected by AgilentTMhigh-throughput miRNA microarray. Results Compared with the control group,there were 26 up-regulated miRNAs（hsa-let-7 b-5 p,hsa-miR-107,hsa-miR-1207-5 p,hsa-miR-1238-3 p,hsa-miR-1246,hsa-miR-1290,hsa-miR-15 a-5 p,hsa-miR-16-5 p,hsa-miR-19 b-3 p,hsamiR-223-3 p,hsa-miR-25-3 p,hsa-miR-320 c,hsa-miR-3663-3 p,hsa-miR-4281,hsa-miR-4455,hsa-miR-4516,hsa-miR-451 a,hsa-miR-4532,hsa-miR-4634,hsa-miR-4687-3 p,hsa-miR-4763-3 p,hsa-miR-486-5 p,hsa-miR-574-5 p,hsa-miR-6090,hsa-miR-6515-3 p,and hsa-miR-92 a-3 p） in the observation group（FC absolute value 2,all P〈0. 05,all FDR〈0. 1）. Conclusion There are 26 differentially expressed miRNAs in the serum of ESCC patients,which may be associated with the occurrence and development of ESCC.

关 键 词：非编码RNA 小分子RNAs 食管肿瘤 食管癌 食管鳞癌 

分 类 号：R735.1[医药卫生—肿瘤]