30只普通绵耳狨猴血液学和血清生化指标的测定及分析  

Detection and analysis of hematological and serum biochemical indices of thirty common marmosets

作　　者：滕永康[1] 丛日旭 刘云波[1] TENG Yongkang;CONG Rixu;LIU Yunbo(Institute of Laboratory Animal Sciences,Chinese Academy of Medical Sciences（CAMS;Comparative MedicineCenter,Peking Union Medical College（PUMC）,Beijing 100021,China)

机构地区：[1]中国医学科学院医学实验动物研究所,北京协和医学院比较医学中心,北京100021

出　　处：《中国比较医学杂志》2018年第5期70-74,共5页Chinese Journal of Comparative Medicine

基　　金：国家重点研发计划(编号：2017YFA0105201); 北京市科技计划(编号：Z161100000216154)

摘　　要：目的检测30只普通绵耳狨猴的血液学、血清生化指标,对雌雄性别组间进行检验,与狨猴和猕猴测定值比对。方法保持动物清醒状态,后肢静脉采血,应用全自动血细胞分析仪及血液生化分析仪分别测定血液学及血清生化指标,测定结果与狨猴和猕猴相关数据进行比较。结果血液学指标中,雌雄组间各指标差异无显著性。血清生化检测中雌猴组与雄猴组差异有显著性的指标有高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)(P<0.05),其他指标差异无显著性,对比国外狨猴群体,本单位饲养的狨猴群体的血红蛋白(HGB)、红细胞平均血红蛋白浓度(MCHC)、中性粒细胞(NEUT)、丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、球蛋白(GLOB)指标数据明显升高,但与猕猴数据测定值范围相符。结论对本单位饲养的普通绵耳狨猴的血液学和血清生化指标进行测定,并与狨猴和猕猴相关数据进行比较,为药理、毒理研究以及狨猴疾病诊疗提供基础数据。Objective To examine hematological and biochemical parameters in 30 common marmosets,count the mean and standard deviations of each index, and analyze significant differences between female and male groups.Additionally,data from marmosets and macaque breeding were compared. Methods Blood was collected through the posterior limb vein while animals were awake. Hematology and serum biochemical indices were then measured with an automatic blood cell analyzer and blood biochemical analyzer,followed by statistical testing. Results No significant differences were measured in hematological indices between male and female groups. There was a significant difference between the female and male group in serum biochemical indices including high-density lipoprotein-cholesterol（HDL-C）and low-density lipoprotein-cholesterol（LDL-C）（P〈0. 05）. Compared with the foreign marmoset group,HGB,MCHC,NEUT,ALT,AST,and GLOB were visibly increased in the group of marmosets fed by our institution,but in accordance with the data range in rhesus monkeys. Conclusions Hematological and serum biochemical indices of commonmarmosets have been detected in this study and compared with related data in macaques and marmosets. Our findings provide basic data not only for pharmacological and toxicological studies,but also diagnosis and treatment of diseases.

关 键 词：普通绵耳狨猴 血液学指标 血清生化指标 

分 类 号：R-33[医药卫生]