正清风痛宁对骨关节炎动物模型ADAMTs-4、ADAMTs-5表达的影响  

Effect of Zhengqingfengtongning on the expression of aggrecanase-1(ADAMTs-4) and aggrecanase-2(ADAMTs-5) in the cartilage tissue of knee joint in rabbit models of osteoarthritis

作　　者：陈志军[1] 张大华[1] CHEN Zhijun;ZHANG Dahua(First Affiliated Hospital of Xi’an Medical University,Xi’an 710077,China)

机构地区：[1]西安医学院第一附属医院,西安710077

出　　处：《中国比较医学杂志》2018年第5期88-94,共7页Chinese Journal of Comparative Medicine

摘　　要：目的分析正清风痛宁对骨关节炎动物模型膝关节软骨组织聚蛋白多糖酶1(ADAMTs-4)、聚蛋白多糖酶2(ADAMTs-5)蛋白表达的影响,探讨正清风痛宁治疗骨关节炎的作用机制。方法将32只成功建模的新西兰兔随机分为观察组和模型组,另选取16只新西兰兔作为空白组。空白组不接受干预治疗,模型组应用生理盐水灌胃,观察组应用正清风痛宁灌胃。药物干预后4周分离膝关节软组织,观察关节形态、关节液、关节软骨,同时进行HE染色和ADAMTs-4、ADAMTs-5 mRNA检测(RT-PCR法),利用统计方法分析组间数据。结果模型组关节软骨分级和Mankin’s评分高于观察组和空白组,观察组关节软骨分级和Mankin’s评分高于空白组(P<0.05)。模型组ADAMTs-4和ADAMTs-5 mRNA表达显著高于观察组和空白组,观察组ADAMTs-4和ADAMTs-5 mRNA表达显著高于空白组(P<0.05)。结论正清风痛宁可改善骨关节炎动物模型关节病变程度,可能与其抑制ADAMTs-4和ADAMTs-5表达有关。Objective To analyze the effect of a Chinese medicine Zhengqingfengtongning on the expression of aggrecanase-1（ADAMTs-4） and aggrecanase-2（ADAMTs-5） in cartilage tissue of knee joint in rabbit models of osteoarthritis,and to study the mechanism of action of Zhengqingfengtongning in treatment for osteoarthritis. Methods 32 New Zealand rabbits were randomly divided into the observation group and the model group,and 16 healthy New Zealand rabbits were chosen as the blank group. The blank group did not receive the intervention treatment,the model group received physiological saline in gastric gavage,the observation group was given the Zhengqingfengtongning by gastric gavage. The soft tissue samples of knee joint in the three groups were taken at 4 weeks after drug intervention. The joint morphology,joint fluid and articular cartilage were observed by histopathology. The ADAMTs-4 and ADAMTs-5 mRNA were detected by RT-PCR. The data were then statistically analyzed. Results The grades of articular cartilage and Mankin＇s score of the model group were significantly higher than those of the observation group and blank group,the gradesof articular cartilage and Mankin＇s score of the observation group were significantly higher than those of the blank group,and the difference between the two groups was statistically significant（P〈0. 05）. The ADAMTs-4 and ADAMTs-5 mRNA expressions of the model group were significantly higher than those of the observation group and blank group. The ADAMTs-4 and ADAMTs-5 mRNA expressions of the observation group were significantly higher than those of the blank group,and the difference between the two groups was statistically significant（P〈0. 05）. Conclusions The Zhengqingfengtongning can improve the degree of joint lesions in osteoarthritis animal models,which may be related to inhibiting the expression of ADAMTs-4 and ADAMTs-5.

关 键 词：正清风痛宁 兔 骨关节炎 聚蛋白多糖酶1 聚蛋白多糖酶2 

分 类 号：R-33[医药卫生]