抑制BCAR1降低肺癌细胞系A549 p-P38表达  

Inhibition of BCAR1 decreases p-P38 expression in lung cancer cell line A549

作　　者：黄伟 阮姝琴 王如文[3] 范小青[3] HUANG Wei;RUAN Sbu-qin;WANG Ru-wen;FAN Xiao-qing(Dept. of Thoracic Surgery, Chongqing General Hospital, Chongqing 400013;Dept. of Oncology and Hematology, Chongqing General Hospital, Chongqing 400013;Center of Thoracic Surgery, Institute of Surgery Research, Daping Hospital the Third Military Medical University, Chongqing 400042, China)

机构地区：[1]重庆市人民医院胸外科,重庆400013 [2]重庆市人民医院肿瘤血液科,重庆400013 [3]第三军医大学大坪医院野战外科研究所全军胸外科中心,重庆400042

出　　处：《基础医学与临床》2018年第6期809-814,共6页Basic and Clinical Medicine

基　　金：重庆市渝中区科技计划项目(20150128)

摘　　要：目的探讨抑制乳腺癌抗雌激素药物耐药蛋白(BCAR1)表达对肺癌细胞系A549 P38和p-P38表达的影响。方法培养正常A549细胞(对照组)、慢病毒BCAR1基因干扰A549细胞(干扰组)和慢病毒阴性对照A549细胞(阴性对照组),用Western blot检测细胞P38和p-P38表达水平,用克隆形成实验、流式细胞计量术、Transwell实验和划痕实验检测细胞的细胞克隆、细胞周期、细胞侵袭和迁移能力。结果干扰组p-P38表达显著低于其他两组(P<0.05);干扰组细胞G1期比例显著高于其他两组(P<0.05);干扰组细胞增殖、侵袭和迁移能力低于其他两组(P<0.05)。结论抑制BCAR1表达可下调p-P38表达,减弱肺癌A549细胞增殖、迁移和侵袭能力。Objective To discuss the effect of breast cancer anti-estrogen resistance 1（ BCAR1） knockdown on the expression of P38 and p-P38 in lung cancer cell line A549. Methods A549 cells（ control group）,A549 cells with RNAi letiviral vector of BCAR1（ interference group） and A549 cells with negative control letiviral vector（ negative group） were cultured. Western blot was used to detect the expression of P38 and p-P38. The proliferation,cell cycle,migration and invasion were measured by colony formation assay,flow cytometer,transwell experiment and scratch adhesion test,respectively. Results p-P38 expression in interference group cells was significantly lower than that in other two group cells（ P〈0. 05）.G1 phase of interference group cells was significantly increasing than that in other two group cells（ P〈0. 05）.The proliferation,migration and invasion of interference group cells were all significantly suppressed as compared with that of other two group cells（ P 0. 05）. Conclusions BCAR1 knockdown decreases p-P38 expression and inhibits proliferation,migration and invasion of A549 cells.

关 键 词：BCAR1 P-P38 P38 A549细胞 肺癌 

分 类 号：R734.2[医药卫生—肿瘤]