高效液相-阳离子交换色谱法测定藏药安神丸中槟榔碱的含量  

Determination of the arecoline content in Tibetan medicine Anshen pills by high performance liquid chromatography-cation exchange chromatography

作　　者：逯雯洁 林鹏程[2] 海平 LU Wen-Jie;LIN Peng-Cheng;HAI Ping(Qinghai Provincial Institute of Drug Control, Key Laboratory for the Study of the Modernization of Tibetan Medicine in Qinghai, Xining 810016, China;College of Chemistry and Life Science, Qinghai University of Nationalities, Xining 810007, China)

机构地区：[1]青海省药品检验检测院,青海省中藏药现代化研究重点实验室,西宁810016 [2]青海民族大学青海民族大学化学与生命科学学院,西宁810007

出　　处：《食品安全质量检测学报》2018年第9期2113-2117,共5页Journal of Food Safety and Quality

基　　金：青海省科技厅项目(2012-N-523)、青海省科技厅科研基础条件平台项目(2017-ZJ-Y40)~~

摘　　要：目的建立高效液相-阳离子交换色谱法测定藏药安神丸中槟榔碱含量的方法。方法样品用乙醚溶液和碳酸盐缓冲液提取后,用Sepax Polysulfonix-SCX(250 mm×4.6 mm,5μm)强阳离子交换树脂色谱柱分离,以乙腈-0.2%磷酸溶液(pH 3.8,10:90,V:V)作为流动相进行洗脱,0.5 mL/min流速,用紫外检测器于215 nm波长处进行测定。结果氢溴酸槟榔碱在7.028~210.840μg/mL范围内线性关系良好(r^2=0.9998),回归方程为Y=44.5058X-56.3314,加标回收率为95.67%~97.41%,相对标准偏差为1.07%~1.80%(n=3)。结论此方法具有良好的精密度、稳定性及回收率,可用于安神丸中槟榔碱成分的测定。Objective To establish a method for determination of the arecoline content in Tibetan medicine Anshen pills by high performance liquid chromatography-cation exchange chromatography. Methods The samples were extracted with a mixture of ether solution and buffer bicarbonate, separated by Sepax Polysulfonix-SCX （250 mm×4.6 mm, 5 μm） with acetonitrile-0.2% phosphoric acid solution （pH3.8, 10：90, V：V as mobile phase for elution, and detected by ultraviolet detector in 215 nm. Results Arecoline had good linearity in the range of 7.028-210.840 μg/mL （Y=44.5058X-56.3314, r2=0.9998）, the average recoveries ranged from 95.67%-97.41%, and the relative standard deviations was 1.07%-1.80% （n=3）. Conclusion This method has good precision, stability and recovery rate, which is suitable for determination of arecoline quantity in Anshen pills.

关 键 词：高效液相色谱法 安神丸 槟榔碱 

分 类 号：O657.7[理学—分析化学] TQ460.72[理学—化学]