高效液相色谱法测定化妆品中酸性黄36等5种组分含量的不确定度评定  

Evaluation of uncertainty in the determination of acid yellow 36 and other 4 kinds of components in cosmetics by high performance liquid chromatography

作　　者：王涛 杨会鸽 李静静 刘鹏鸣 杜晖 范菲 郑研 WANG Tao;YANG Hui-Ge;LI Jing-Jing;LIU Peng-Ming;DU Hui;FAN Fei;ZHENG Yan(xi ＇an Institute for Food and Drug Control, Xi ＇an 710054, China)

机构地区：[1]西安市食品药品检验所,西安710054

出　　处：《食品安全质量检测学报》2018年第9期2181-2187,共7页Journal of Food Safety and Quality

摘　　要：目的对《化妆品安全技术规范》(2015年版)中高效液相色谱法测定酸性黄36、颜料橙5、颜料红53:1、苏丹红Ⅱ和苏丹红Ⅳ5种禁用物质含量进行不确定度评定。方法根据JJF 1059.1-2012《计量技术规范》,建立数学模型,考察不确定度来源并给出了量化结果,计算出合成标准不确定度和扩展不确定度。结果当取置信概率为95%,包含因子k=2,被测化妆品中5种物质各含量为ω_(酸性黄36)=510.1μg/g、ω_(颜料橙5)=525.3μg/g、ω_(颜料红53:1)=546.4μg/g、ω_(苏丹红)Ⅱ=498.6μg/g和ω_(苏丹红)Ⅳ=476.8μg/g时,其相应标准扩展不确定度分别为56.2μg/g、57.8μg/g、60.2μg/g、54.9μg/g和52.4μg/g。结论本方法给评定测量结果的可靠性提供科学依据,并为优化测量过程提出了改进建议。Objective Base on Safety and Technical Standards for Cosmetics （2015 Edition）, the uncertainty of determination of 5 prohibited substances （acid yellow 36, pigment orange 5, pigment red 53：1, sudan Ⅱ and sudan Ⅳ） in cosmetics was evaluated by high performance liquid chromatography （HPLC）. Methods According to JJF1059-2012 Measurement Technical Standard, the mathematical model was established, the source of uncertainty was investigated and quantified results were given, and the uncertainty of the synthesis standard and the uncertainty of the extension were calculated. Results The expanded uncertainties were 56.2 μg/g, 57.8 μg/g, 60.2 ktg/g, 54.9 μg/g and 52.4 μg/g （95% confidence level, k=-2） when the content of 5 prohibited substances （acid yellow 36, pigment orange 5, pigment red 53： l, Sudan Ⅱ and Sudan Ⅳ） in cosmetics were 510.1 μg/g, 525.3 μg/g, 546.4 μg/g, 498.6 μg/g and 476.8 μg/g. Conclusion This method can provide basis for evaluating the reliability of the measurement results, and put forward some suggestions for improving the measurement process.

关 键 词：高效液相色谱法 化妆品 禁用物质 不确定度 

分 类 号：O657.72[理学—分析化学] TQ658[理学—化学]