贝那普利和氨氯地平对自发性高血压大鼠促胰液素、生长抑素表达的影响  

The influence of benazepril and amlodipine on the expression of secretin and somatostatin in spontaneously hypertensive rats

作　　者：金华[1,2] 刘志军 颜春鲁[1] 刘峰林[1] 陈丽[1] 张秋菊[1] 徐厚谦[1] 胡继宏[1] 窦荣海[1] 温鑫洋[1] JIN Hua;LIU Zhi-jun;YAN Chun-lu;LIU Feng-lin;CHEN Li;ZHANG Qiu-ju;XU Hou-qian;HU Ji-hong;DOU Rong-hai;WEN Xin-yang(Gansu University of Chinese Medicine,Lanzhou 730000;Laboratory of Herbs Mining and Innovation and Transformation of Traditional Chinese Medicine in Gansu Province,Lanzhou 730000,China)

机构地区：[1]甘肃中医药大学,兰州730000 [2]甘肃省中医方药挖掘与创新转化重点实验室,兰州730000

出　　处：《中国应用生理学杂志》2018年第2期154-158,I0001,共6页Chinese Journal of Applied Physiology

基　　金：国家自然科学基金资助项目(81160477);甘肃省自然科学研究基金计划(1107RJZA220);甘肃省高等学校科研项目协同创新团队项目(2017C-14)

摘　　要：目的:探讨贝那普利和氨氯地平对自发性高血压大鼠(SHR)促胰液素(PZ)、生长抑素(SS)表达的影响。方法:14周龄雄性SHR 45只随机分为3组(n=15):SHR组、贝那普利组(盐酸贝那普利灌服0.90 mg·kg^(-1)·d^(-1))、氨氯地平组(苯磺酸氨氯地平灌服0.45 mg·kg^(-1)·d^(-1))。以Wistar-Kyoto大鼠(WKY)作为正常对照组(n=15),SHR组及WKY组每日灌服等体积蒸馏水。干预8周后,采用免疫组化法和RT-PCR法检测十二指肠中PZ、胃窦中SS蛋白和mRNA表达的变化。结果:干预8周后,与WKY组比较,SHR组十二指肠PZ、胃窦SS蛋白和mR-NA表达明显升高(P<0.05);与SHR组比较,贝那普利组与氨氯地平组十二指肠PZ蛋白和mRNA、胃窦SS蛋白和mRNA表达均明显降低(P<0.05);与贝那普利组比较,氨氯地平组十二指肠PZ蛋白和mRNA、胃窦中SS mR-NA表达降低更为明显(P<0.05)。结论:SHR十二指肠中PZ和胃窦中SS调节失衡,贝那普利和氨氯地平的降压作用可能通过调节PZ和SS的表达而达到降压目的,而氨氯地平的调节作用较显著。Objective：Investigate the influence of benazepril and amlodipine on the expression of secretin（PZ）and somatostatin（SS）in spontaneously hypertensive rats（SHR）.Methods：Forty-five SHRs（14 weeks old,male）were randomly assigned into 3groups（n=15）：SHR group,Benazepril group（which was given benazepril 0.90 mg·kg^-1·d^-1）and Amlodipine group（SHRs were given amlodipine 0.45 mg·kg^-1·d^-1）,taking Wistar-Kyoto（WKY）as normal control（n=15）,meanwhile,rats in SHR group and WKY group were given the same volume of distilled water.After 8 weeks of intervention,the expression of protein and mRNA of PZ in duodenum and SS in sinuses ventriculi was detected by enzyme-linked immunoassay and RT-PCR.Results：After 8 weeks of intervention,compared with the WKY group,the expression of protein and mRNA of PZ in duodenum and SS in sinuses ventriculi was increased significantly in SHR group（P〈0.05）.Compared with SHR group,the expression of PZ in duodenum and SS in sinuses ventriculi was decreased significantly in Benazepril group and Amlodipine group（P〈0.05）.Compared with Benazepril group,in Amlodipine group the expression of PZ mRNA in duodenum and SS mRNA in sinuses ventriculi was decreased more significantly（P〈0.05）.Conclusion：The regulation disorder of PZ in duodenum and SS in sinuses ventriculi exists in SHR.The antihypertensive effect of benazepril and amlodipine may be realized by regulating the expression of PZ and SS,while the regulation of amlodipine is more obvious than benazepril.

关 键 词：自发性高血压大鼠 促胰液素 生长抑素 贝那普利 氨氯地平 

分 类 号：R363[医药卫生—病理学]