Nd：YAG激光对牙龈卟啉单胞菌的杀菌作用探讨  

An investigation on antibacterial effects of Nd:YAG laser on the Porphyromonas gingivalis

作　　者：吴一凡[1] 宋忠臣[1] 束蓉[1] WU Yi-fan;SONG Zhong-chen;SHU Rong(Department of Periodontology , Ninth People＇s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Lab of Stomatology, Shanghai 200011, China)

机构地区：[1]上海交通大学医学院附属第九人民医院牙周科、上海市口腔医学重点实验室,上海200011

出　　处：《牙体牙髓牙周病学杂志》2018年第5期258-264,302,共8页Chinese Journal of Conservative Dentistry

基　　金：国家自然科学基金(81570977)

摘　　要：目的:观察Nd:YAG激光对牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)的杀菌效果。方法:培养P.gingivalis并置备其生物膜。结晶紫染色后观察功率为6 W的Nd:YAG激光不同照射时间对浮游状态下P.gingivalis成膜能力的影响;荧光染色后分别用激光共聚焦显微镜、扫描电镜观察Nd:YAG激光不同照射时间对P.gingivalis单菌种生物膜活力和结构的影响。结果:Nd:YAG激光照射35 s时,对浮游状态下P.gingivalis的成膜能力有抑制作用;照射45 s时,对生物膜状态下P.gingivalis活力产生抑制。生物膜的结构及生物膜中细菌的结构,随着光照时间延长受到破坏的程度不断增加,主要破坏形式为菌体的汽化消失。结论:Nd:YAG激光能够抑制浮游状态下P.gingivalis的成膜能力,对单一菌种生物膜中的细菌活力和细菌结构产生影响,表明Nd:YAG激光可用于牙周炎辅助治疗。AIM： To investigate the antibacterial effects of Nd：YAG laser against Porphyromonas gingivalis（ P.gingivalis） in vitro. METHODS： P.gingivalis were in vitro cultured and P.gingivalis biofilm was prepared. The cultured P.gingivalis were treated by Nd：YAG laser at 6 W for 0 ~ 60 s respectively. The biofilm formation ability of planktonic P.gingivalis was observed by crystafl violet test; the viability of P.gingivalis cells in mature biofilms was detected by confocal Laser scanning microscopy（ CLSM）; the morphology of mature biofilms was observed by scanning electron microscopy（ SEM）. RESULTS： The biofilm formation ability of P.gingivalis was limted under 35 s laser radiation. The viability of P.gingivalis in mature biofilm went down under 45 s iradiation. The deformation of mature biofilm and the deconstruction of becterium became distincted with the iradiation time increased. The major destructional form was evaporation. CONCLUSION： Nd：YAG laser may inhibit of biofilm formation of planktonic P.gingivalis and destroy the construction of the mature biofilm and bacteria.

关 键 词：牙周致病菌 单一菌种生物膜 ND:YAG激光 体外实验 

分 类 号：R780.2[医药卫生—口腔医学]