结直肠癌患者结肠黏膜肿瘤坏死因子α诱导蛋白8样因子3的表达与意义  

Expression and its clinical significance of tumor necrosis factor-α-induced protein-8 like-3 in colonic mucosaof patients with colon cancer

作　　者：范燕云[1] 肖传兴 刘文明[1] 林振和[1] 任建林[1] 许鸿志[1] Fan Yanyun;Xiao Chuanxing;Liu Wenming;Lin Zhenhe;Ren J ianlin;Xu Hongzhi(Department of Gastroenterology, Zhongshan Hospital, Xiamen University, Fujian Province 361004, China)

机构地区：[1]厦门大学附属中山医院消化内科,福建省361004

出　　处：《中华消化杂志》2018年第3期171-176,共6页Chinese Journal of Digestion

基　　金：厦门市重要重大疾病联合攻关项目（3502220149031）;厦门市科技计划项目（3502220154020）

摘　　要：目的分析结直肠癌患者结肠黏膜肿瘤坏死因子α诱导蛋白8样因子3（TIPE3）的表达异常与临床病理特征之间的相关性。 方法采用实时荧光定量PCR检测58份结直肠癌组织及其癌旁组织中TIPE3 mRNA的表达情况，采用免疫组织化学SP法检测83份结直肠癌组织及其癌旁组织中TIPE3蛋白质的表达情况。采用非参数秩和检验和卡方检验进行统计学分析。 结果结直肠癌组织中TIPE3 mRNA相对表达量为0.719（0.104，0.887），低于癌旁组织的4.770（1.732，6.800），差异有统计学意义（Z＝－6.345，P〈0.05）。TIPE3 mRNA在不同性别、年龄和TNM分期的结直肠癌组织中表达差异均无统计学意义（P均〉0.05），有淋巴结转移组TIPE3 mRNA表达量低于无淋巴结转移组[0.113（0.061, 0.375）比0.489（0.327，0.956）；Z＝－3.815，P〈0.01]，术后生存期〈5年的患者TIPE3 mRNA表达量低于术后生存期≥5年患者组[0.104（0.049, 0.220）比0.482（0.266, 0.908）；Z＝－3.653，P〈0.01]，术后复发组患者TIPE3 mRNA表达量低于术后未复发组[0.188（0.091, 0.493）比0.409（0.233, 1.010）；Z＝－2.431，P＝0.015]。TIPE3 mRNA高表达组结直肠癌患者术后5年复发率低于TIPE3 mRNA低表达组[23.1%（6/26）比56.2%（18/32）]，差异有统计学意义（χ2＝6.508，P〈0.05）。结直肠癌组织中TIPE3蛋白质阳性表达率低于癌旁组织[44.6%（37/83）比68.7%（57/83）；χ2＝8.004，P〈0.05]，TIPE3的蛋白质表达在不同年龄、性别中差异均无统计学意义（P均〉0.05），Ⅱ期患者TIPE3蛋白质阳性表达率高于Ⅲ期患者[60.5%（23/38）比29.7%（11/37）]，差异有统计学意义（χ2＝7.174，P〈0.05），有淋巴结转移组TIPE3蛋白质阳性表达率低于无淋巴结转移组[28.2%（11/39）比59.1%（26/44）]，差异有统计学意义（χ2＝7.983，P＝0.005）。 结论TIPE3在结直肠癌组织中的表达较癌旁组织降低，且与结直肠癌的淋�ObjectiveTo detect the expression of tumor necrosis factor-α-induced protein-8 like-3 （TIPE3） in colonic mucosa of patients with colon cancer, and to analyze the correlation between its abnormal expression and clinicopathological features of patients with colon cancer. MethodsThe expression of TIPE3 mRNA in 58 cases of colon cancer and tumor-adjacent tissues was detected by real-time polymerase chain reaction （RT-PCR）. The expression of TIPE3 at protein level in 83 cases of colon cancer and tumor-adjacent tissues was determined by SP immunohistochemistry. Nonparametric rank-sum test and chi-square test were performed for statistical analysis. ResultsThe relative expression of TIPE3 mRNA in the colon cancer tissues was 0.719 （0.104 to 0.887）, which was lower than that of tumor-adjacent tissues （4.770, 1.732 to 6.800）, and the difference was statistically significant （Z=-6.345, P〈0.05）. There was no statistically significant difference in the expression of TIPE3 mRNA in colon cancer tissues between different gender, age and TNM stage （all P〉0.05）. The expression of TIPE3 mRNA in group of patients with lymph node metastasis （0.113, 0.061 to 0.375） was lower than group of patients without lymph node metastasis （0.489, 0.327 to 0.956; Z=-3.815, P〈0.01）. The expression of TIPE3 mRNA of patients survived less than five years after operation （0.104, 0.049 to 0.220） was lower than that of patients survived over five years （0.482, 0.266 to 0.908; Z=-3.653, P〈0.01）. The expression of TIPE3 mRNA of patients with recurrence after operation （0.188, 0.091 to 0.493） was lower than that of patients without recurrence （0.409, 0.233 to 1.010; Z=-2.431, P=0.015）. The recurrence rate of TIPE3 mRNA high expression group in five years after operation was lower than that of TIPE3 mRNA low expression group （23.1%, 6/26 vs 56.2%, 18/32）; and the difference was statistically significant （χ2=6.508, P〈0.05）. The expression of TIPE3 at protein level of colon cancer tiss

关 键 词：结直肠肿瘤 肿瘤转移 肿瘤坏死因子α诱导蛋白8样因子3 

分 类 号：R735.34[医药卫生—肿瘤]