RSK4对结直肠癌细胞增殖的影响  

Effect of RSK4 on Proliferation of Colorectal Cancer Cells

作　　者：邓野 蔡君[1] 王炫 王梦 张森[1] 杨永华 喻世华 付国权[1] 杨继元[1] DENG Ye;CAI Jun;WANG Xuan(First Affiliated Hospital of Yangtze University ,Jingzhou 434000, Chin)

机构地区：[1]长江大学附属第一人民医院

出　　处：《肿瘤学杂志》2018年第4期341-345,共5页Journal of Chinese Oncology

基　　金：2015年湖北省卫计委一般项目(WJ2015MB212)

摘　　要：[目的]探讨RSK4（p90 ribosomalprotein S6 kinase4）在结直肠癌细胞的表达情况及其生物学作用。[方法]挑选结直肠癌细胞株SW480和HCT116,用Lipofectamin 2000技术将RSK4基因转染至细胞核内,建立结直肠癌RSK4基因高表达细胞系,用RT-PCR及WB证实了RSK4在结直肠癌细胞SW480和HCT116转染成功,采用MTT、流式细胞仪技术检测转染RSK4后对结直肠癌细胞增殖的影响。[结果]RSK4在结直肠癌细胞呈低表达,转染成功后对结直肠癌细胞有明显的抑制作用,并使S期细胞所占的比例下降,G0/G1期比例上升（P〈0.05）。[结论 ]RSK4基因在结直肠癌细胞株中呈低表达,转染RSK4后可抑制肿瘤细胞生长,有可能为一个潜在的抑癌基因。[Objective] To investigate the effects of RSK4（P90 Ribosomal Protein S6） in colorectal cancer cells. [Methods] RSK4 gene was transfected into colorectal cancer SW480 and HCT116 cells by Lipofectamin 2000. The expression of RSK4 in wild and transtected SW480 and HCT116 cells was confirmed by RT-PCR and Western blot. Cell proliferation was detected by MTT and flow cytometry. [Results] RSK4 was lowly expressed in wild SW480 and HCT116 cells,while RSK4 was over-expressed in transfected cells. MTT assay showed that the proliferation was significantly inhibited in RSK4-transfected colorectal cancer cells. Flow cytometry revealed that S phase cells decreased and G0/1 cells increased significantly in transfected colorectal cancer cells（P〈0.05）. [Conclusion] Low expression of RSK4 gene is detected in colorectal cancer cells,and transfection of RSK4 inhibits cancer cell proliferation,which indicates that RSK4 may be a tumor suppressor gene in colorectal cancer.

关 键 词：RSK4 结直肠肿瘤 抑癌基因 

分 类 号：R735.37[医药卫生—肿瘤]