法舒地尔抑制肺肌成纤维细胞分化的作用机制  

THE INHIBITING EFFECT OF FASUDIL ON MYOFIBROBLAST DIFFERENTIATION FROM LUNG FIBROBLAST

作　　者：刘鑫[1] 张倩 王馥丽[2] 常延河[1] 刘丽[1] 李宏芬[1] Liu Xin;Zhang Qian;Wang Fuli(Clinical Laboratory, Kailuan General Hospital, Tangshan , 06300)

机构地区：[1]开滦总医院检验科,河北省唐山市063000 [2]开滦总医院病理科

出　　处：《中国煤炭工业医学杂志》2018年第2期163-165,共3页Chinese Journal of Coal Industry Medicine

基　　金：河北省卫计委医学科学研究重点课题计划项目(编号:20150848)

摘　　要：目的观察法舒地尔对大鼠肺成纤维细胞向肌成纤维细胞分化的抑制作用。方法培养原代新生鼠肺成纤维细胞,予以转化生长因子(TGF)-a1诱导向肌成纤维细胞分化,并予以法舒地尔预处理。免疫组织化学染色法检测a-平滑肌肌动蛋白(SMA)的表达。免疫印迹法检测I型胶原(Col I)、血清反应因子(SRF)和a-SMA的表达。结果免疫组织化学染色结果显示,给予TGF-a1刺激,能够显著诱导其向肌成纤维细胞分化,胞浆内出现明显的肌丝样结构。免疫印迹结果显示,与对照组比较,TGF-a1诱导组Col I、SRF和a-SMA表达显著上调,分别是对照组的6.25倍、4.50倍和3.58倍;而法舒地尔预处理组与TGF-a1诱导组比较,Col I、SRF和a-SMA的表达量是TGF-a1诱导组的36.00%、35.56%和38.87%,差异均有统计学意义(P<0.05)。结论法舒地尔能够抑制TGF-a1介导的肺肌成纤维细胞分化。Objective To explore the inhibiting effect of Fasudil on myofibroblast differentiation from lung fibroblast.Methods Myofibroblast differentiation was induced by transform growth factor（TGF）-a1 from lung fibroblast of neonatal rat.The positive of a-smooth muscle actin（a-SMA）was test by immunohistochemical（IHC）staining.The expression of collage type I（Col I）,serum response factor（SRF）and a-SMA were measured by western blot.Results The IHC staining results showed that positive of a-SMA can be seen in myofibroblast induced by TGF-a1,and reduced by pre-treatment of Fasudil.Compared with control,the expression of Col I,SRF and a-SMA were increased to 6.25 fold,4.50 fold,and 3.58 fold,respectively.Pre-treated with Fasudil,the expression of Col I,SRF and a-SMA were decreased to 36.00%,35.56%,and38.87%,respectively.Conclusion Myofibroblast differentiation induced by TGF-a1 can be inhibited by Fasudil treatment.

关 键 词：法舒地尔 肺成纤维细胞 Rho相关卷曲螺旋形成蛋白激酶 转化生长因子-a1 A-平滑肌肌动蛋白 I型胶原 血清反应因子 

分 类 号：R363[医药卫生—病理学]