TNF-α调控牙周膜干细胞骨向分化中LncRNA的差异表达  

Differential expression of LncRNA during osteogenic differentiation of periodontal ligament stem cells regulated by TNF-α

作　　者：崔诗曼 张清彬[1] 左志向 曹威[1] 赖世翔 CUI Shiman;ZHANG Qingbin;ZUO Zhixiang;CAO Wei;LAI Shixiang(510140, Stomatology Hospital of Guangzhou Medical University, China;Cancer Center of Sun Yat-sen University, Guangzhou)

机构地区：[1]广州医科大学附属口腔医院,510140 [2]中山大学肿瘤防治中心

出　　处：《实用口腔医学杂志》2018年第3期318-322,共5页Journal of Practical Stomatology

基　　金：广东省科技厅科技计划项目(编号:201511013;2016ZC0147);广东省荔湾区科信局项目(编号:20151217049)

摘　　要：目的:探讨肿瘤坏死因子-α(TNF-α)对人牙周膜干细胞(PDLSCs)成骨作用的影响,以及其中长链非编码(LncRNA)的差异表达情况。方法:分离培养PDLSCs,对照组细胞用成骨诱导液培养,实验组细胞用成骨诱导液+10 ng/ml TNF-α培养。通过茜素红染色、RT-PCR和Western bolt检测两组细胞骨向分化,用高通量测序技术探究LncRNA的差异表达情况。结果:在TNF-α作用下,PDLSCs的骨向分化能力减弱;测序结果发现差异表达显著的长链非编码RNA上调的有57条,下调的有26条。结论:LncRNA可能与PDLSCs骨向分化的调控密切相关。Objective： To study the osteogenic differentiation of periodontal ligament stem cells（PDLSCs） regulated by tumor necrosis factor-α（TNF-α） and the differential expression of long noncoding RNA（LncRNA）. Methods： PDLSCs were cultured by osteogenic induction medium with and without TNF-α respectively. The osteogenic differentiation ability of PDLSCs were tested by Alizarin red staining, RT-PCR and Western blot. The differential expression of LncRNA was explored by high-throughput sequencing. Results： TNF-α inhibited osteogenic differentiation potential of PDLSCs. There were 57 up-regulated and 26 down-regulated LncRNAs in PDLSCs treated by TNF-α. Conclusion： LncRNA may be closely related to the regulation of osteogenic differentiation of PDLSCs.

关 键 词：牙周膜干细胞(PDLSCs) 肿瘤坏死因子-α(TNF-α) 长链非编码RNA(LncRNA) 骨向分化 炎症 

分 类 号：R781.4[医药卫生—口腔医学]