CXCL12/CXCR4生物学轴及AMD3100对舌鳞癌Tca8113细胞侵袭行为的影响  

The effects of CXCL12/CXCR4 biological axis and AMD3100 on the invasion of Tca8113 cells

作　　者：王钢 董国伟 WANG Gang;DONG Guowei(471000, Luoyang Central Hospital Affiliated to Zhengzhou University, China)

机构地区：[1]郑州大学附属洛阳中心医院,471000

出　　处：《实用口腔医学杂志》2018年第3期400-403,共4页Journal of Practical Stomatology

摘　　要：目的:研究探讨CXCL12/CXCR4生物学轴和CXCL12抑制剂AMD3100对癌细胞侵袭行为的影响。方法:将体外培养的Tca8113细胞分为4组:对照组细胞常规培养,CXCL12组(CX组)附加终浓度100 ng/ml的CXCL12培养细胞,AMD3100组(AM组)附加终浓度为1μg/ml的AMD3100培养细胞,干预组(AM+CX组)以1μg/ml的AMD预处理1 h后加入100ng/ml CXCL12培养细胞,各组细胞培养48 h。逆转录PCR检测细胞CXCR4受体mRNA表达;用Transwell小室检测细胞侵袭力。结果:对照组、CX组、AM组、AM+CX组CXCR4 mRNA相对表达量分别为0.514±0.057、0913±0.018、0.486±0.053和0.260±0.023;细胞穿膜数分别为43.6±2.3、75.1±2.9、42.5±2.5和32.2±1.9。结论:CXCR4受体在舌鳞癌Tca8113细胞阳性表达;CXCL12能促进Tca8113细胞侵袭力,AMD3100可抑制其迁移。Objective： To study the influence of CXCL12/CXCR4 biological axis and CXCL12 inhibitor AMD3100 on the invasion of tongue squamous cell carcinoma Tca8113 cells. Methods： Tca8113 cells were conventionally cultured （control group）, cultured with the addition of CXCL12 at 100 ng/ml（CX group）, AMD3100 at 1μg/ml（AMD group） and AMD（1 μg/ml） for 1 h followed by CXCL12（ 100 ng/ml） （AM ＋ CX group） respectively. The cells in all groups were eutured for 48 h. CXCR4 mRNA expression of the cells was examined by RT-PCR. The invasion of the cells was examined by transwell with matrigel. Results： CXCR4 was positively expressed in Tea8113 cells. The relative expression in control, CX, AM and AM ＋ CX gropus was 0. 514 ±0.057, 0. 913 ±0. 018, 0.486 ± 0. 053 and 0. 260 ± 0. 023, the cells invaded through transwell membrane were 43.6 ±2.3, 75.1 ± 2.9, 42.5 ± 2.5 and 32. 2± 1.9, respectively. Conclusion： CXCL12/CXCR4 biological axis plays an important role in the invasion of Tca8113 cells.

关 键 词：CXCL12/CXCR4 侵袭 TCA8113细胞 AMD3100 

分 类 号：R739.8[医药卫生—肿瘤]