拉沙病毒IgG抗体间接免疫荧光检测方法的建立  

Development of indirect immunofluorescence assay for Lassa virus IgG detection

作　　者：李深伟[1] 杨柳[1] 李帅[1] 张子龙[1] 张晓航[1] 田桢干[1] 李平[1] LI Shen-wei, YANG Liu, LI Shuai, ZHANG Zi-long, ZHANG Xiao-hang, TIAN Zhen-gan, LI Ping(Shanghai Entry-Exit Inspection and Quarantine Bureau, Shanghai 200335, Chin)

机构地区：[1]上海出入境检验检疫局,上海200335

出　　处：《中国国境卫生检疫杂志》2018年第2期81-83,F0003,共4页Chinese Journal of Frontier Health and Quarantine

基　　金：国家重大传染病专项(2013ZX10004803);国家质检总局科技计划项目(2016IK215;2017IK008)

摘　　要：目的建立简便、快速的拉沙病毒IgG抗体检测方法。方法利用293F细胞表达重组拉沙病毒糖蛋白GP1,固相化在腔室玻片上,加入GP1抗血清,最后加入荧光标记的二抗,利用间接免疫荧光法检测拉沙病毒IgG抗体。结果制备了重组拉沙病毒糖蛋白GP1,GP1抗血清效价>1∶1000000;拉沙病毒IgG抗体间接免疫荧光方法具有专一性强(检测20份健康人血清,无交叉反应),灵敏度较高(抗血清在1∶1000稀释时仍有较强信号)的特点。结论建立了拉沙病毒IgG抗体的快速间接免疫荧光检测方法,可用于口岸拉沙病毒的快速筛查。Objective To develop an indirect immunofluorescence assay for Lassa virus antibody detection. Methods Recombinant Lassa virus glycoprotein 1（GP1） was expressed on 293 F cells and immobilized on the chamber slide.Rabbit serum containing polyclonal antibodies against Lassa virus GP1 was developed and added on chamber slide,and then FITC conjugated second antibody was added. Lassa virus IgG could be detected by the indirect immunofluorescence assay in this study. Results GP1 recombinant proteins were purified and certified by Western blot. The titer of GP1 antibody was more than 1 ∶ 1000000. The indirect immunofluorescence assay for Lassa virus antibody had the advantage of high specificity（no cross reactivity,tested in 20 health human serum samples） and high sensitivity（with strong positive signals even in more than 1000 times dilution）. Conclusion This study had developed the fast immunofluorescence assay of Lassa virus antibody with the big advantages of high specificity and sensitivity.

关 键 词：拉沙病毒 免疫荧光法 真核细胞 抗体 

分 类 号：R183.7[医药卫生—流行病学]