Caveolin-1基因敲除小鼠下颌第一磨牙的表型观察  

Phenotypic Observation of Mandibular First Molar in Caveolin-1 Gene Knockout Mice

作　　者：史璐[1] 田菲菲 白书锋 冯海玲 SHI Lu;TIAN Fei-fei;BAI Shu --feng;FENG Hai -lin.(Department of Endodontics, the First Affiliated Hospital of Zhengzhou versity , School of Stomatology , Zhengzhou University. Zhengzhou 450000, China)

机构地区：[1]郑州大学第一附属医院郑州大学口腔医学院牙体牙髓科,河南郑州450000

出　　处：《口腔医学研究》2018年第5期480-484,共5页Journal of Oral Science Research

基　　金：国家自然科学基金(编号：U1204813);河南省高校重点科研项目(编号：18B310030)

摘　　要：目的:观察Caveolin-1基因敲除小鼠(knockout,KO)下颌第一磨牙牙胚组织学形态及牙齿表型,为深入研究Caveolin-1在牙齿发育中的作用提供组织学基础。方法:经聚合酶链式反应(polymerase chain reaction,PCR)鉴定基因型后,HE染色观察不同发育阶段的KO小鼠及同源野生型(wildtype,WT)小鼠的下颌第一磨牙牙胚组织学形态;显微CT观察其牙体硬组织结构。结果:胚胎14.5d,WT鼠牙胚发育进入帽状期,但KO鼠牙胚仍处于蕾状期,成釉器未见明显分层及原发性釉结形成。胚胎16.5d,KO鼠成釉器出现细胞分层,但成釉器体积较小,颈环处上皮芽向下方间充质中深入较浅。胚胎18.5d,KO鼠成釉细胞和成牙本质细胞的细胞极化不明显,且成牙本质细胞缺乏典型柱状形态。出生后1个月,下颌第一磨牙显微CT及三维重建结果显示:KO鼠与WT鼠牙体解剖形态未见明显差异。结论:Caveolin-1基因表达缺失可导致小鼠下颌第一磨牙牙胚发育蕾状期-帽状期过渡出现暂时性滞后,但对牙齿后期发育无明显影响。Objective：To investigate the histological morphology of mandibular first molar tooth germ and analyse dental phenotype in caveolin-1 gene knockout（KO）mice.Methods：After genotype identification with polymerase chain reaction（PCR）,mandibular first molar germs and teeth were collected from caveolin-1 gene knockout mice and wild-type（WT）homologous mice.The histological morphology of tooth germs and dental phenotype were studied by hematein eosin（HE）staining and micro computed tomography（Micro-CT）,respectively.Results：At E14.5,the tooth germ of WT mouse was at the cap stage;however,the tooth germ of KO mouse was still at the bud stage,and no obvious stratification and primary enamel knot were found.With the tooth germ development,the enamel organ cells of KO mouse stratified into four layers at cap stage（E16.5）.While,enamel organ of KO mouse had smaller volume and less invagination of cervical loop into epithelial mesenchymal.At E18.5,the cell polarities of both ameloblasts and odontoblasts were not obvious in KO tooth germ.And the odontoblasts were lack of typical high columnar shapes.The results of Micro-CT and 3 Dreconstruction showed that no significant difference was found between the two mice＇s lower first molar anatomic shape.Conclusion：The deficiency of caveolin-1 gene led to temporal arrest for the bud-to-cap transition in developmental molar tooth germs without significant influence on the later developmental processes.

关 键 词：CAVEOLIN-1 牙齿发育 MICRO-CT 表型观察 

分 类 号：R782.1[医药卫生—口腔医学]