聚左旋丙交酯纳米纤维联合骨髓间充质干细胞修复大鼠脊髓损伤的效果  

Effect of implantation of PLLA nanofibers and BMSCs on spinal cord injury of rats

作　　者：高锋[1] 张晓峰[1] 段艳伟[1] 严怀宁[1] 潘永飞[1] 叶荣[1] GAO Feng;ZHANG Xiaofeng;DUAN Yanwei;YAN Huaining;PAN Yongei;YE Rong.(Orthopaedic Department, Nanjing Hospital, Jiangsu Provincial Corps, Chinese People＇s Armed Police Force, Nanjing 210028,China)

机构地区：[1]武警江苏总队南京医院骨科,210028

出　　处：《武警医学》2018年第2期138-142,共5页Medical Journal of the Chinese People's Armed Police Force

摘　　要：目的探讨聚左旋丙交酯纳米纤维联合骨髓间充质干细胞移植修复大鼠脊髓损伤的效果。方法在体外,制备材料和提取干细胞后,将骨髓间充质干细胞种植于聚左旋丙交酯纳米纤维材料上,通过CCK-8法观察材料上干细胞的增殖情况,在维甲酸的诱导分化后,通过光镜和免疫组织化学方法观察材料上干细胞的神经分化。在体内,制备成大鼠脊髓损伤的模型后,分别移植有干细胞黏附的聚左旋丙交酯纳米纤维膜(联合组)、聚左旋丙交酯纳米纤维膜(纤维膜组)、骨髓间充质干细胞(干细胞组),以及空白植入(空白对照组)。术后2、4、8周对大鼠进行BBB功能评分,观察大鼠脊髓损伤修复的效果。结果体外研究观察发现,在聚左旋丙交酯纳米纤维材料上的干细胞与普通培养皿中的相比,第1、4天未见明显差异,第7天材料上的干细胞增殖好于普通培养皿中的。在纤维材料上的干细胞向神经样细胞的诱导分化也好于普通培养皿中的。体内实验发现,术后4周,联合组、纤维膜组与干细胞组之间未见明显差异,均优于空白对照组,术后8周,联合组优于纤维膜组及干细胞组,且均优于空白对照组。结论聚左旋丙交酯纳米纤维联合骨髓间充质干细胞移植修复大鼠脊髓损伤效果良好。Objective To explore the effect of implantation of PLLA nanofibers and BMSCs on spinal cord injury of rats.Methods In vitro,PLLA nanofibers were fabricated and BMSCs were isolated before BMSCs were implanted on the PLLA nanofibers.The Cell Counting Kit-8 was applied to detect the proliferation of BMSCs.The neural differentiation of BMSCs on the PLLA nanofibers induced by RA（retinoic acid） was observed using immunohistochemistry and light microscopy.After the spinal cord injury（SCI） model was prepared,forty-eight rats were randomly and equally assigned to four groups（n = 12） ：the PLLA nanofibers ＋ BMSCs group,PLLA nanofibers group,BMSCs group,and the blank control group.The motor functional recovery of rats was evaluated with the BBB score at2 w,4 w,8 w after operation.Results There was no significant change in the proliferation of BMSCs on PLLA nanofibers compared with culture dishes at 1-day and 4-day.The proliferation of BMSCs on PLLA nanofibers was better than in culture dishes at 7-day.The neural differentiation of BMSCs on PLLA nanofibers was better than in culture dishes.In vivo,there was no significant difference in the rats＇ functional recovery after SCI between the PLLA nanofibers ＋ BMSCs group,the PLLA nanofibers group and the BMSCs group at4-week after operation.The rats＇ functional recovery of the PLLA nanofibers ＋ BMSCs group was better than that of the PLLA nanofibers group and the BMSCs group at 8-week after operation.The rats＇ functional recovery of the above three groups was better than that of the blank control group at 4-week and 8-week after operation.Conclusions The implantation of PLLA nanofibers and BMSCs can promote the recovery of spinal cord injury of rats.

关 键 词：聚左旋丙交酯纳米纤维 骨髓间充质干细胞 脊髓损伤 神经分化 

分 类 号：R744[医药卫生—神经病学与精神病学]