日本血吸虫感染过程中糖酵解途径对调节性T细胞产生的影响  

Effects of glycolytic pathway on generation of regulatory T cells during Schistosoma japonicum infection

作　　者：王小番 齐倩倩 张丽娜 许磊[1] 董利阳 李娅琳 濮亚男 韦川 周莎[1] 朱继峰 陈晓军[1] 苏川[1] WANG Xiao-fan;QI Qian-qian;ZHANG Li-na;XU Lei;DONG Li-yang;LI Ya-lin;PU Ya-nan;WEI Chuan;ZHOU Sha;ZHU Ji-feng;CHEN Xiao-jun＇;SU Chuan(Department of Pathogen Biology, Nanjing Medical University, Nanjing 211166, China)

机构地区：[1]南京医科大学病原生物学系,南京211166

出　　处：《中国血吸虫病防治杂志》2018年第2期131-135,共5页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81430052;81501765);江苏省自然科学基金(BK20150995)

摘　　要：目的探索日本血吸虫感染过程中糖酵解途径对小鼠调节性T(Treg)细胞数量和功能的影响。方法建立日本血吸虫感染小鼠模型,用糖酵解抑制剂2-Deoxy-D-glucose(2DG)或PBS对日本血吸虫感染小鼠进行6次腹腔注射后,分离脾脏细胞和肠系膜淋巴结,采用流式细胞术(FCM)检测分离得到的细胞中Glut1^+CD4^+T细胞以及Treg细胞比例。结果未感染组小鼠脾脏(43.58%±2.50%vs.21.15%±0.96%;t=8.834,P<0.01)和肠系膜淋巴结中Glut1^+CD4^+T细胞比例(38.97%±1.97%vs.28.40%±2.11%;t=3.662,P<0.05)均显著高于感染日本血吸虫8周小鼠,但未感染组小鼠脾脏(6.83%±0.21%vs.13.30%±0.35%;t=15.65,P<0.01)和肠系膜淋巴结中Treg细胞比例(8.26%±0.15%vs.14.37%±0.44%;t=13.14,P<0.01)均显著低于感染组小鼠。感染小鼠给与2DG腹腔注射后,脾脏(15.50%±0.76%vs.13.07%±0.15%;t=3.130,P<0.05)和肠系膜淋巴结中Treg细胞比例(17.00%±0.41%vs.13.83%±0.18%;t=6.947,P<0.01)显著高于给与PBS注射小鼠。结论糖酵解途径抑制了日本血吸虫感染小鼠Treg细胞分化。Objective To assess the influence of glycolytic pathway on the proportion and numbers of regulatory T cells during Schistosoma japonicum infection.Methods A S. japonicum-infected mouse model was established,and C57/BL6 male mice infected with S. japonicum were subjected to intraperitoneal injections of with the glycolytic inhibitor 2-Deoxy-D-glucose（2 DG）or PBS for 6 times,and then the cells from spleen or mesenteric lymph nodes（LNs）were isolated and analyzed by flow cytometry（FCM）to detect the percentage of Glut1＋CD4＋T cells and Treg cells.Results The proportions of Glut1＋CD4＋T cells in the spleen（43.58%±2.50% vs. 21.15%±0.96%;t = 8.834,P〈 0.01）and mesenteric LNs（38.97%±1.97% vs. 28.40%±2.11%;t = 3.662,P〈 0.05）were higher in the normal mice than those in the infected mice,and the percentages of Treg cells in the spleen（6.83%±0.21% vs. 13.30%±0.35%;t = 15.65,P 〈0.01）and LNs（8.26%±0.15% vs. 14.37%±0.44%;t =13.14,P 〈0.01）were lower in the normal mice than those in the infected mice. In addition,the proportions of Treg cells in the spleen（15.50%±0.76% vs. 13.07%±0.15%;t = 3.130,P〈 0.05）and LNs（17.00% ± 0.41% vs. 13.83% ± 0.18%;t = 6.947,P〈 0.01）were higher in the infected mice injected intraperitoneally with 2 DG than those in the infected mice injected intraperitoneally with PBS.Conclusion Glycolytic pathway inhibits Treg differentiation in the spleen and mesenteric LNs of S. japonicum-infected mice.

关 键 词：日本血吸虫 糖酵解途径 GLUT1 TREG细胞 

分 类 号：R383.24[医药卫生—医学寄生虫学]