Production of Free Amino Acid and Short Peptide Fertilizer from Rapeseed Meal Fermentation Using Bacillus flexus NJNPD41 for Promoting Plant Growth  

作　　者：LIU Hongjun ZHONG Xi HUANG Yan QIAO Cece SHAO Cheng LI Rong SHEN Qirong 

机构地区：[1]Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Jiangsu Collaborative Innovation Center for Solid Organic Waste Resource Utilization, National Engineering Research Center for Organic-based Fertilizers, Nanjing Agricultural University

出　　处：《Pedosphere》2018年第2期261-268,共8页土壤圈（英文版）

基　　金：supported by the National Key Research and Development Program of China (Nos. 2016YFE0101100 and 2016YFD0200106);the National Key Basic Research Program of China (No. 2015CB150506);the Fundamental Research Funds for the Central Universities of China (Nos. KYZ201519 and BK20160710);the Jiangsu Science and Technology Program of China (No. BY2016077-05);the Jiangsu Provincial Natural Science Foundation of China (Nos. BK20150059 and BK20160710);the Top-notch Academic Programs Project of Jiangsu Higher Education Institution, China (No. PPZY2015A061)

摘　　要：Free amino acids and short peptides(FAPS)that play an important role in plant nutrition can be extracted from rapeseed meal(RSM)by microbial enzymolysis.The enzymolytic activity of one bacterial strain isolated from RSM for the production of FAPS fertilizer(FAPSF)via solid-state fermentation,as well as its detoxification activity for isothiocyanates and oxazolidinethione,was investigated in this study.The strain NJNPD41 isolated from RSM piled-soil possessed effective proteolytic activity and was identified as Bacillus flexus on the basis of its morphological,physiological,and biochemical properties,as well as 16S rDNA analysis.Compared to the uninoculated control,inoculation with the strain NJNPD41 significantly increased the yield of total free amino acids and short peptides by 115%and decreased the content of isothiocyanates and oxazolidinethione by 53%and 60%,respectively,after solid-state fermentation.The pot and field experiments showed that FAPSF significantly promoted eggplant growth and enhanced the fruit yield and quality of eggplant compared to the control.In conclusion,this study provided a novel method for the high-value utilization of RSM to produce high-quality and low-toxicity FAPSF using the newly isolated strain B.flexus NJNPD41.Free amino acids and short peptides(FAPS)that play an important role in plant nutrition can be extracted from rapeseed meal(RSM)by microbial enzymolysis.The enzymolytic activity of one bacterial strain isolated from RSM for the production of FAPS fertilizer(FAPSF)via solid-state fermentation,as well as its detoxification activity for isothiocyanates and oxazolidinethione,was investigated in this study.The strain NJNPD41 isolated from RSM piled-soil possessed effective proteolytic activity and was identified as Bacillus flexus on the basis of its morphological,physiological,and biochemical properties,as well as 16S rDNA analysis.Compared to the uninoculated control,inoculation with the strain NJNPD41 significantly increased the yield of total free amino acids and short peptides by 115%and decreased the content of isothiocyanates and oxazolidinethione by 53%and 60%,respectively,after solid-state fermentation.The pot and field experiments showed that FAPSF significantly promoted eggplant growth and enhanced the fruit yield and quality of eggplant compared to the control.In conclusion,this study provided a novel method for the high-value utilization of RSM to produce high-quality and low-toxicity FAPSF using the newly isolated strain B.flexus NJNPD41.

关 键 词：bacterial strain DETOXIFICATION activity ISOTHIOCYANATE microbial ENZYMOLYSIS N POOL organic FERTILIZER solid-statefermentation oxazolidinethione 

分 类 号：S154.4[农业科学—土壤学]