金线莲多糖协同ConA对小鼠脾淋巴细胞分泌Th1、Th2型细胞因子及其mRNA表达量的影响  

Regulatory Effects of Anoectochilus roxburghii Polysaccharide(ARP) on ConA-induced Cytokines Secretion and mRNA Expression by Murine Splenocytes

作　　者：马玉芳 郑小香 郑乃珍 李健[2] 秦韬[2] 黄一帆[2] Ma Yufang;Zheng Xiaoxiang;Zheng Naizhen;Li Jian;Qin Tao;Huang Yifan(University Key Laboratory for Integrated Chinese Traditional and Western Veterinary Medicine and Animal Healthcare in Fujian Province, Fuzhou 350002;Fujian Key Laboratory of Traditional Chinese Veterinary Medicine and Animal Health （Fujian Agriculture and Forestry University）, Fuzhou 350002)

机构地区：[1]中西兽医结合与动物保健福建省高校重点实验室,福州350002 [2]福建省兽医中药与动物保健重点实验室(福建农林大学),福州350002

出　　处：《中国食品学报》2018年第4期72-78,共7页Journal of Chinese Institute Of Food Science and Technology

基　　金：国家自然科学基金项目(31372474)

摘　　要：目的:研究金线莲多糖(ARP)调节Con A刺激小鼠脾淋巴细胞细胞因子分泌水平及其m RNA表达影响,探讨其免疫调节作用机制。方法:小鼠脾淋巴细胞经不同质量浓度的金线莲多糖协同Con A体外刺激,ELISA法检测小鼠脾淋巴细胞上清液中Th1(IL-2、IFN-γ)、Th2(IL-4、IL-6)细胞因子的含量;q RT-PCR检测上述细胞因子及其转录因子T-bet、GATA-3 m RNA表达量。结果:在试验质量浓度范围,ARP能显著提高小鼠脾淋巴细胞Th1、Th2细胞因子的分泌量,促进Th1、Th2细胞因子及转录因子(T-bet、GATA-3)m RNA表达(P<0.01)。结论 :ARP能协同Con A促进小鼠脾淋巴细胞分泌Th1、Th2型细胞因子及其m RNA表达,从而发挥免疫调节的作用,作用机制可能与上调核转录因子T-bet、GATA-3的m RNA表达水平有关。Objective： To study regulatory effects of Anoectochilus roxburghii polysaccharide（ARP） on ConA-induced cytokines secretion and mRNA expression by splenocyte in mice and its possible mechanism. Methods ： Murine splenocytes were treated with ConA and different mass concentrations of ARP, the levels of Th1 cytokines（IL-2, IFN-γ） and Th2 cytokines（IL-4, IL-6） in supernatant was determined by ELISA. The mRNA levels of aboved cytokines and transcript factors（T-bet and GATA-3） were measured by quantitative real-time polymerase chain reaction（q RT-PCR）. Results： ARP（50-400 μg/mL） increased significantly ConA-stimulated Th1 and Th2 cytokines production of splenocytes possessed, promoted above cytokines as well as transcript factors T-bet and GATA-3 mRNA expression. Conclusion： The results showed that ARP could induce Th1 cytokines（IL-2, IFN-γ） and Th2 cytokines（IL-4, IL-6） gene expression followed by Th1, Th2 cytokines production in murine splenic lymphocytes via up regulating Th1 and Th2 transcript factors T-bet and GATA-3 mRNA expression.

关 键 词：金线莲多糖 脾淋巴细胞 细胞因子 mRNA 

分 类 号：R285.5[医药卫生—中药学]