脐血CD_3AK细胞和其培养上清诱导HL-60凋亡  

HL-60 Cells' Apoptosis Induced by Cord Blood's CD_3AKCells and CD-3AK Cultural Supernatants

作　　者：何秉燕[1] 张渝侯[1] 陈冬珍 邹典定[1] 李小明[2] 王捷荣[1] 

机构地区：[1]湖北医科大学附属第二医院儿科,430071 [2]湖北医科大学生化教研室

出　　处：《天津医药》2000年第12期714-716,F004,共4页Tianjin Medical Journal

基　　金：湖北省重点科技发展计划资金资助课题

摘　　要：目的:探讨脐血CD_3AK细胞和其培养上清(CM)诱导HL-60细胞凋亡的作用。方法:用细胞形态学观察,DNA琼脂糖电泳,原位末端标记法分别检测出HL-60自然凋亡与CD_3AK细胞诱导和CM诱导的HL-60细胞凋亡率。结果:HL-60细胞自然凋亡率为(4.60±2.17)%;CD_3AK细胞诱导HL-60细胞凋亡率为(21.35±4.46)%(t=6.80,P<0.01),CM诱导的HL-60细胞凋亡率为(33.08±5.12)%(t=8.26,P<0.01)。结论:脐血CD_3AK细胞和其培养上清能诱导HL-60凋亡。Objective:To explore HL-60 cells' apoptosis induced by cord blood's CD3AK cells and CD3AK cultural supernatants (CM). Methods:HL-60 cells' natural apoptosis and that induced by cord blood's CD3AK cells and CD3 AK cultural super-natants were detected by TUNEL technique,cell morphology,and DNA agarose gel electrophoresis respectively. Results:The natural apoptosis rate of HL-60 cells was (4.60± 2.17)% , the rate of HL-60 cells' apoptosis induced by CD3AK was (21.35±4.46)%(t = 6.80,P<0.01),and that induced by CM was (33.08 ?5.12) %(t = 8.26,P<0.01). Conclusion: HL-60 cells' apoptosis can be induced by cord blood's CD3AK cells and CM.

关 键 词：T淋巴细胞亚群 CD3抗原 胎血 脱噬作用 生物疗法 髓性白血病 细胞凋亡 

分 类 号：R733.7[医药卫生—肿瘤]