48例非小细胞肺癌MDR_1及MRP基因表达结果分析  

Analysis of Expression of Multidrug Resistance Gene and Multidrug Resistance-Associated Protein Gene in Patients with Non-small-cel I Lung Cancer

作　　者：张广明[1] 田建立[1] 

机构地区：[1]天津医科大学总医院老年病研究室,300052

出　　处：《天津医药》2000年第12期738-739,共2页Tianjin Medical Journal

摘　　要：目的:探讨多药耐药(MDR_1)基因、多药耐药相关蛋白(MRP)基因在非小细胞肺癌(NSCLC)中的表达情况及其与组织类型的关系。方法:用逆转录多聚酶链反应(RT-PCR)方法检测新鲜肺癌组织标本。结果:48例NSCLC标本MDR_1、MRP基因阳性表达率分别为62.5%、66.7%,二种基因共同表达率为43.8%。肺鳞癌MRP基因表达明显高于腺癌(P<0.01),而鳞癌与腺癌之间MDR_1基因表达无显著性差异(P>0.05)。结论:MDR_1、MRP基因可以共同或分别存在于肺癌组织中,肺鳞癌MRP基因表达率较腺癌高。Objective:To investigate the expression of multidrug resistance (MDR1 ) gene and multidrug resistance-associated protein (MRP) gene in patients with non-small-cell lung cancer (NSCLC) and its relation to histopathological type. Methods: The MDR1 and MRP genes were determined by reverse transcription-polymerase chain reaction (RT-PCR) in 48 NSCLC specimens.Results:The positive rates of the MDR1 and MRP gene expression were 62.5% and 66.7% respectively in the 48 patients. Twenty-one of the 48 NSCLCs (43.8%) expressed both MDR1 and MRP genes. The positive rates of the MRP gene expression in squamous cell carcinoma was significantly higher than those in adenocarcinoma (P < 0.01 ) ,and there was no significant difference in MDR1 gene expression between the two groups (P >0.05). Conclusion: The MDR1 and MRP gene expressions were both or respectively in the NSCLCs. The incidence of the MRP gene expression in squamous cell carcinoma was higher than that in adenocarcinoma.

关 键 词：非小细胞肺癌 MRP基因 肺肿瘤 基因表达 聚合酶链反应 MDR1基因 NSCLC 

分 类 号：R734.2[医药卫生—肿瘤]