氧化胁迫对粉红螺旋聚孢霉生长和产厚垣孢子的影响  

Effects of Oxidative Stress on Hyphal Growth and Chlamydospore Formation of Clonostachys rosea

作　　者：王琦[1,2] 冯静红 孙占斌 姜维治[2] 李世东 孙漫红[2] 马桂珍[1] WANG Qi;FENG Jing-hong;SUN Zhan-bin;JIANG Wei-zhi;LI Shi-dong;SUN Man-hong;MA Gui-zhen(School of Marine Science and Technology, Huaihai Institute of Technology, Lianyungang 222005;Institute of Plant Protection Chinese Academy of Agricultural Sciences, Belting 100193)

机构地区：[1]淮海工学院,连云港222005 [2]中国农业科学院植物保护研究所,北京100193

出　　处：《生物技术通报》2018年第4期168-173,共6页Biotechnology Bulletin

基　　金：国家自然科学基金项目(31471815);国家重点研发计划(2017YFD0201102);现代农业产业体系(CARS-25-B-02)

摘　　要：通过改变液体发酵通气量和添加不同种类活性氧(O2-,H_2O_2,OH·),研究氧化胁迫对粉红螺旋聚孢霉67-1菌丝生长和产厚垣孢子的影响。结果表明,氧化胁迫对真菌菌丝和孢子的影响不同。低氧胁迫下,67-1菌丝生长状态发生改变,培养液装量越多,菌丝分枝越少,并且厚垣孢子产量越低。发酵过程中添加一定量的活性氧可以调控粉红螺旋聚孢霉厚垣孢子的形成。接种前添加5-6 mmol/L H_2O_2,菌株产厚垣孢子水平显著提高,浓度达到9 mmol/L时,67-1生长和产孢完全受到抑制。培养16 h后添加,67-1对H_2O_2的耐受力增强。培养基中添加甲萘醌能够抑制厚垣孢子的形成,培养16 h后添加350μmol/L甲萘醌则可以提高67-1厚垣孢子的产量。在Fe2+-H_2O_2体系中,不同添加时间高氧胁迫均能够显著促进厚垣孢子的生成(P<0.05)。The effects of oxidative stress on hyphal growth and chlamydospore production in Clonostachys rosea 67-1 was investigatedthrough adjusting ventilatory volume and adding various kinds of reactive oxygen（O-（2-）,H2O2 and OH·）. The results showed that the effectsof oxidative stress on fungal mycelia were different from that on chlamydospores. When aeration rate was low,the growth status of the funguschanged;with the increasing of loading volume,the branches of mycelia reduced,and the yield of chlamydospores decreased sharply.Reactive oxygen at certain amount affected the formation of chlamydospores during 67-1 fermentation. When 5-6 mmol/L H2O2 was added beforeinoculation,the production of chlamydospores significantly increased,and the growth and sporulation of 67-1 were completely inhibited whilethe concentration was 9 mmol/L. The oxygen-tolerance of the fungus was remarkably enhanced when H2O2 was supplied at 16 h after inoculation.Menadione inhibited chlamydospore formation when added into the fermentation liquor directly,but adding 350 μmol/L menadione significantlypromoted fungal sporulation after incubated for 16 h. In Fe-（2＋）-H2O2 system,high oxygen stress in different time significantly increasedchlamydospore production（P 0.05）.

关 键 词：粉红螺旋聚孢霉 氧化胁迫 活性氧 厚垣孢子 真菌生长 

分 类 号：TQ927[轻工技术与工程—发酵工程]