蓝光对龙眼细胞培养及类黄酮代谢的影响  

Effects of Blue Light on the Cultivation of Longan Cells and Flavonoid Metabolism

作　　者：李汉生[1] 姚德恒 陈晓慧[1] 刘炜婳[1] 陈裕坤[1] 林玉玲[1] 王云 赖钟雄[1] LI Hansheng;YAO Deheng;CHEN Xiaohui;LIU Weihua;CHEN Yukun;LIN Yuling;WANG Yun;LA! Zhongxiong(Institute of Horticultural Biotechnology, Fujian Agriculture and Forestry University, Fuzhou, Fujian 350002, Chin)

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002

出　　处：《热带作物学报》2018年第4期694-701,共8页Chinese Journal of Tropical Crops

基　　金：国家自然科学基金(No.31572088、No.31672127); 福建省重大专项(No.2015NZ0002-1); 福建省自然科学基金杰出青年项目(No.2015J06004)

摘　　要：采用搅拌式生物反应器放大培养龙眼悬浮细胞,探讨蓝光对龙眼细胞生长及类黄酮积累的影响。基于已建立并优化的龙眼细胞悬浮培养体系,首先研究龙眼细胞在黑暗和蓝光的培养过程中,细胞生长量、类黄酮含量、细胞活力、培养液的底物消耗量等的变化情况。结果发现:龙眼细胞培养9 d后,蓝光的细胞干重比黑暗增长了0.28 g/L,类黄酮含量增长了0.77 mg/g。细胞培养前期蓝光的培养液蔗糖消耗速度慢于黑暗培养,此后蔗糖含量均稳定在2 g/L。培养过程中,蓝光培养的还原糖含量均高于黑暗培养,蓝光的磷酸盐的消耗量基本大于黑暗培养。其次,通过qPCR技术分析光信号转录因子DlHY5、调控基因DlPAP1及类黄酮途径合成基因DlCHS的表达差异。结果表明蓝光可能通过光信号转录因子DlHY5调控基因DlPAP1的表达,进而调控龙眼类黄酮代谢途径合成基因DlCHS的表达,从而导致类黄酮的积累。The effects of blue light on the growth of longan cells and flavonoid accumulation were investigated using a stirred bioreactor with enlarged culture of longan cells in this study. Based on the established and optimized suspension culture system of longan cells, the changes of the growth of cells, flavonoid contents, cell vitality, substrate consumption and so on were firstly studied in the dark and blue light treatments. Results found that the dry weight of blue light increased by 0.28 g/L and the flavonoid contents increased by 0.77 mg/g after 9 days culture. The sucrose consumption under blue light was slower than that under the darkness in the early stages of culture, and then the sucrose contents were stable at 2 g/L. In the process of culture, the contents of reducing sugar under blue light was higher than that under darkness, and the phosphate consumption under blue light was more than that under darkness. Then, the expression of light signal transcription factor DlHY5, regulatory gene DlPAP1 and flavonoid biosynthesis pathway structural gene DlCHS were analyzed by qPCR. Results indicated that the regulation of flavonoid accumulation in longan by blue light is probably through the light signal transcription factor DlHY5, and then regulated the expression of flavonoid biosynthesis pathway regulatory gene DlPAP1 and structural genes DlCHS.

关 键 词：龙眼 细胞培养 生物反应器 蓝光 类黄酮 

分 类 号：S667.2[农业科学—果树学]