血浆微RNA-216早期预警重症急性胰腺炎的临床价值  

The clinical value of plasma microRNA-216 for early identifying the severity of acute pancreatitis

作　　者：邓力珲[1] 张晓鑫[1] 陈炜炜[2] 石娜[1] 马芸 姜坤[1] 杨晓楠[1] 夏庆[1] DENG Lihui;ZHANG Xiaoxin;CHEN Weiwei;SHI Na;MA Yun;JIANG Kun;YANG Xiaonan;XlA Qing(Department of Integrated Traditional and Western Medicine, West China Hospital, Sichuan University, Chengdu, Sichuan 610041, P. R. China;Department of Gastroenterology, Northern Jiangsu People＇s Hospital, Yangzhou, ]iangsu 225001, P. R. China)

机构地区：[1]四川大学华西医院中西医结合科,成都610041 [2]苏北人民医院消化内科,江苏扬州225001

出　　处：《华西医学》2018年第5期550-554,共5页West China Medical Journal

基　　金：国家自然科学基金(81300358)

摘　　要：目的探讨血浆微RNA-216(microRNA-216,miR-216)早期评估重症急性胰腺炎(severe acute pancreatitis,SAP)的临床价值。方法 2014年9月-11月纳入发病48 h内入院的急性胰腺炎患者,采用实时定量聚合酶链反应法检测血浆中miR-216的表达,比较不同程度急性胰腺炎患者血浆miR-216表达水平的差异,分析其早期诊断SAP的价值。结果共纳入25例患者SAP患者血浆的miR-216Ct值(32.40±1.43)高于轻症急性胰腺炎(35.85±1.91,P<0.05)和中度重症急性胰腺炎(35.90±2.44,P<0.05),而轻症急性胰腺炎和中度重症急性胰腺炎患者之间miR-216表达差异无统计学意义(P>0.05)。血浆miR-216诊断SAP的受试者工作特征曲线下面积为0.792(P<0.05),与血C反应蛋白、尿素氮、炎症因子等临床常用的指标相比较,在诊断SAP方面差异无统计学意义(P>0.05)。结论血浆miR-216早期诊断SAP的价值不优于目前临床上常用的评价指标体系,尚需大样本的临床试验予以证实。Objective To investigate the value of plasma microRNA-216（miR-216） in patients with acute pancreatitis as a clinical biomarker to early identify severe acute pancreatitis（SAP）. Methods Patients with acute pancreatitis who admitted to the hospital within 48 hours after the onset of disease between September and November2014 were enrolled in this study. Plasam and clinical data of all the patients were collected. MiR-216 in the plasma was detected using quantitative real time-polymerase chain reaction. Results A total of 25 patients were enrolled. The Ct value of plasma miR-216 in SAP patients（32.40±1.43） was significantly upregulated than mild acute pancreatitis（MAP）（35.85±1.91, P〈0.05） and moderately severe acute pancreatitis（MSAP） patients（35.90±2.44, P〈0.05）, respectively. The area under receiver operating characteristic curve for plasma miR-216 in predicting SAP was 0.792（P〈0.05）, which did not differ much from other conventional parameters such as C-reactive protein, urinary nitrogen, and cytokines（P〉0.05）.Conclusion Plasma miR-216 is significantly upregulated in SAP patients compared with MAP and MSAP, but it shows no inferior efficiency than the investigated conventional predictors in predicting SAP.

关 键 词：急性胰腺炎 重症急性胰腺炎 微RNA-216 预测指标 生物标志物 

分 类 号：R576[医药卫生—消化系统]