肝细胞癌患者中NLRC5的表达及其临床意义  

Expression of NLRC5 in hepatocellular cacinoma and its clinical significance

作　　者：汪欢[1] 俞广进[1] 熊奇如[1] WANG Huan;YU Guang-jin;XlONG Qi-ru.(The First Hospital of Anhui Medical University, Hefei 230022, Chin)

机构地区：[1]安徽医科大学第一附属医院,合肥230022

出　　处：《肝胆外科杂志》2018年第2期149-153,共5页Journal of Hepatobiliary Surgery

摘　　要：目的探讨核苷酸结合结构域受体家族含CARD结构域-5(NLRC5)在肝细胞癌组织中表达情况及与临床病理特征、预后的关系。方法采用免疫组化SP法检测94例肝细胞癌组织及其癌旁正常组织中NLRC5的表达;Western blot检测20例新鲜肝细胞癌组织及其对应的癌旁正常组织中NLRC5的表达。结果 NLRC5在肝细胞癌组织中的阳性率(62.77%)显著高于癌旁正常组织(19.15%,P<0.01);NLRC5的表达与肝细胞癌分化程度、TNM分期、有无硬化有关(P<0.05),与患者年龄、性别、AFP值、肿瘤大小、HBV感染、肿瘤包膜是否完整无关(P>0.05)。肝细胞癌组织中蛋白相对表达量(0.280±0.049)明显高于相应癌旁正常组织中(0.038±0.009,P<0.01)。NLRC5阳性患者的3年生存期明显低于阴性患者(P<0.01),并且NLRC5为肝细胞癌预后的独立性危险因素。结论 NLRC5蛋白在肝细胞癌组织中高表达,且与患者的临床病理特征、预后等因素有关,可能在肝细胞癌发生、发展过程中起着重要作用,有望成为肝细胞癌新的肿瘤标志物和潜在的治疗靶点。Objective To explore the expression of nucleotides-binding domain receptor family containing CARD domain-5 （ NLRC5 ） in hepatoceUular cacinoma （HCC） tissues, and to analysis the correlation between its expression and clinicopathological and prognosis of HCC. Methods The expression of NLRC5 was examined in 94 cases of HCC tissues and its adjacent normal peritumoral tissues by immunohistochemistry of SP. Westter blot was used to detect the expression of NLRC5 in 20 cases fresh HCC tissues and the corresponding normal peritumoral tissues. Results The expression of NLRC5 was signficantly higher in HCC tissues （62.77%） compared with normal peritumoral tissues（ 19. 15% ,P 〈0. 01 ）. The positive expression of NLRC5 was related to the differentiation of hepatocellular carcinoma, TNM stage and cirrhosis. However, there was no correlation with age, sex,tumor size, HBV infection and tumor capsule（P 〉 0. 05）. The relative expression levels of NLRC5 protein was also significantly higher in HCC tissues （0. 280 ±0. 049 ） comparaed with corresponding normal peritumoral tissues（0. 038±0. 009 ,P 〈 0. 01 ）. The 3-years overall survival of HCC patients with NLRC5 expression was significantly lower than that of patients without NLRC5 expression （ P 〈 0.01 ）, and NLRC5 is an independent prognotie factor for HCC. Conclusion NLRC5 protein is highly expressed in hepatoeellular carcinoma and is associated with the elinicopathological features, prognosis and other factors. NLRC5 is an independent risk factor for prognosis of hepatoeellular carcinoma and may play an important role in the occurrence and development of hepatoeellular carcinoma, It is expected to become a new tumor marker and potential therapeutic target for hepatocellular earcinoma.

关 键 词：肝细胞癌 NLRC5 免疫组化学 WESTERN BLOT 预后 

分 类 号：R735.7[医药卫生—肿瘤]