有氧运动联合高压氧对全脑缺血再灌注大鼠认知功能、血脑屏障及氧自由基的影响  

Effects of aerobic exercise combined with hyperbaric oxygen on cognition, blood brain barrier and oxygen free radicals after cerebral isehemia reperfusion in rats

作　　者：田锋[1] 胡力旬[2] Tian Feng;Hu Lixun(Department of Neurology of Jining First People＇s Hospital, Jining 272000, Chin)

机构地区：[1]济宁市第一人民医院神经内科,山东省济宁272000 [2]济宁医学院基础学院病理生理学教研室

出　　处：《中华航海医学与高气压医学杂志》2018年第2期81-85,共5页Chinese Journal of Nautical Medicine and Hyperbaric Medicine

基　　金：山东省医药卫生科技发展计划项目（2016WSA08026）

摘　　要：目的探讨有氧运动预处理结合高压氧（hyperbaric oxygen，HBO）对全脑缺血再灌注大鼠认知功能、血脑屏障及氧自由基代谢的影响。方法75只雄性Sprague-Dawley大鼠按照数字表法随机分成假手术组、模型组、有氧运动预处理组、HBO治疗组和有氧运动预处理＋HBO治疗组。采用改良Pulsinelli四血管闭塞法构建大鼠全脑缺血再灌注模型。水迷宫测试检测各组大鼠的认知功能，采用伊文思蓝（Evans blue, EB）定量和Zo-1的表达水平评价各组大鼠脑屏障通透性，羟胺法测定大鼠脑组织海马区超氧化物歧化酶（superoxide dismutase，SOD）的活性，硫代巴比妥酸法测定丙二醛（malondialdehyde，MDA）水平。结果同假手术组相比，模型组大鼠认知功能受损（P〈0.05）; EB浓度和Zo-1表达水平升高，SOD活性降低[（47.5±5.7）mmol/mg vs. （24.5±3.8） mmol/mg]，且MDA含量升高[（0.9±0.1） mmol/mg vs. （1.7±0.4） mmol/mg]（均P〈0.05）；有氧运动预处理和HBO治疗均能显著改善大鼠的认知能力，降低EB浓度、Zo-1表达水平和MDA含量（均P〈0.05），并提高SOD活性（均P〈0.05），尤以有氧运动预处理联合HBO组效果最佳（P〈0.01）。结论全脑缺血再灌注大鼠模型构建之前对大鼠进行适度规律性有氧运动辅以HBO处理可显著改善全脑缺血性大鼠的认知功能和血脑屏障，抑制其氧化应激，尤以有氧运动＋HBO处理组效果最佳。ObjectiveTo investigate the effects of aerobic exercise pre-treatment combined with hyperbaric oxygen （HBO） on cognition, blood brain barrier and oxygen free radicals after cerebral isehemia reperfusion in rats.MethodsSeventy-five male Sprague-Dawley rats were randomly divided into 5 groups： the sham group, the model group, the aerobic exercise pre-treatment group, the HBO group, and the aerobic exercise pretreatment＋ HBO group, each consisting of 15 animals. The cerebral ischemia-reperfusion model was established by using the modified Pulsinelli four-blood-vessel-occlusion method. The water maze test was used to evaluate the cognitive function of rats in each group, and the Evans blue （EB） method and Zo-1 expression level were used to evaluate blood brain barrier permeability, and the activity of superoxide dismutase （SOD） and malondialdehyde （MDA） level in hippocampus were measured with the hydroxylamine method and TBA method. ResultsThe cognitive function of the rats in the model group was impaired, as compared with that of the sham group（P〈0.05）, EB concentration and the expression level of Zo-1 were increased, and SOD activity was decreased and MDA level was increased（P〈0.05）. Aerobic exercise pre-treatment plus HBO exposure could significantly improve the cognitive function of rats, reduce the concentration of EB, as well as the expression level of Zo-1. Furthermore, it could increase SOD activity, and and MDA concentration, plus best effects were achieved in the aerobic exercise pretreatment＋ hyperbaric oxygen treatment group（P〈0.01）. ConclusionsModerate or proper aerobic exercise combined with HBO therapy before establishment of the cerebral ischemia-reperfusion model could significantly improve cognitive function and blood brain barrier, inhibit oxidative stress, and best effects were seen in the aerobic exercise pretreatment＋ hyperbaric oxygen treatment group.

关 键 词：有氧运动 高压氧 血脑屏障 氧自由基 全脑缺血再灌注 大鼠 

分 类 号：R743[医药卫生—神经病学与精神病学]