microRNA-145调控Catenin-δ 1表达及其与结直肠癌侵袭和转移的相关性  

MicroRNA-145 suppresses tumour invasion and metastasis in colorectal cancer through regulating Catenin-δ1

作　　者：叶伟标[1,2] 徐咏强 李妤玲 卢碧燕 杨湘玲 刘焕亮[2,3] 李仲均 YE Wei-biao;XU Yong-qiang;LI Yu-ling;LU Bi-yan;YANG Xiang-ling;LIU Huan-liang;LI Zhong-jun(Dongguan Hospital of Southern Medical University, Dongguan, Guangdong 523059;Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor Diseases, Guangzhon, Guangdong 510655;The Sixth Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510655, China)

机构地区：[1]南方医科大学附属东莞人民医院,广东东莞523059 [2]广东省结直肠盆底疾病研究重点实验室,广东广州510655 [3]中山大学附属第六医院,广东广州510655

出　　处：《热带医学杂志》2018年第5期582-586,共5页Journal of Tropical Medicine

基　　金：国家自然科学基金(81702399)

摘　　要：目的探讨microRNA-145(miR-145)调控Catenin-δ1表达与结直肠癌侵袭和转移的关系。方法应用实时荧光定量PCR和显色原位杂交技术检测36例结直肠癌组织和配对正常肠粘膜组织中miR-145的表达;利用LipofectamineTM2000试剂将miR-145模拟物瞬时转染SW620肠癌细胞株,通过RT-PCR和Westernblot分析Catenin-δ1及Fascin-1表达情况;CCK-8试剂盒和Transwell小室检测各组细胞转染后增殖、侵袭和迁移能力的变化。结果miR-145在结直肠癌组织中的表达低于配对正常肠粘膜组织,且表达水平与淋巴结转移相关(P<0.05)。体外细胞实验显示,转染miR-145模拟物可显著降低SW620肠癌细胞株Catenin-δ1和Fascin-1蛋白表达水平,并抑制肿瘤细胞增殖、侵袭和迁移。结论miR-145低表达与结直肠癌侵袭和转移相关,其机制可能与调控Catenin-δ1的表达水平相关。Objective To investigate the role of micro RNA-145（miR-145）in colorectal cancer（CRC）invasion and metastasis. Methods The expression levels of miR-145 were determined by real-time quantitative PCR and in situ hybridization（ISH）in 36 CRC tissues and matched adjacent normal mucosa. miR-145 mimics and siRNA were transiently transfected into human colorectal cancer cells to up-regulate or down-regulate gene expression,and their effects on cell migration and invasion were analyzed by Transwell assay. Cell proliferation was measured using the Cell Counting kit-8. The expression of Catenin-δ1 and Fascin-1 was examined by Western blot. Results miR-145 was down-regulated in CRC tissues,and low expression of miR-145 was significantly associated with lymph node metastasis（P〈0.05）. Transfection of mi R-145 mimics inhibited proliferation,invasion and migration in SW620 cells. Furthermore,up-regulation of miR-145 inhibited the expression levels of Catenin-δ1 and Fascin-1. Conclusion miR-145 played an important role in mediating invasive and metastatic behavior in CRC,probably through regulating the expression of Catenin-δ1.

关 键 词：microRNA-145 Catenin-δ 1 FASCIN-1 结直肠癌 

分 类 号：R735.3[医药卫生—肿瘤]