EGCG通过PTEN增强ATRA对早幼粒白血病细胞株的分化作用  

Epigallocatechin-3-gallate Promotes All-trans Retinoic Acid-induced Maturation of Leukemia Cells via PTEN

作　　者：姚仕菲 刘北忠[1,2] 陈敏 赵毅 李连文 刘路 徐婷[1] 单志灵 肖春兰[1] 淦柳根 钟梁[2] Yao Shifei;Liu Beizhong;Chen Min;Zhao Yi;Li Lianwen;Liu Lu;Xu Ting;Shan Zhiling;Xiao Chunlan;Gan Liugen;Zhong Liang(Central Laboratory, Yongchuan Hospital of Chongqing Medical University, Chongqing, 402160;Key Laboratory of Chongqing, Key Laboratory of Medical Diagnostics of Ministry of Education, Department of Laboratory Medicine, Chongqing Medical University, Chongqing, 400016)

机构地区：[1]重庆医科大学附属永川医院中心实验室,重庆402160 [2]重庆医科大学临床检验诊断学教育部重点实验室、重庆市重点实验室,重庆400016

出　　处：《基因组学与应用生物学》2018年第5期2182-2186,共5页Genomics and Applied Biology

基　　金：国家自然科学基金(81171658);重庆市自然科学基金(2011BA5037)共同资助

摘　　要：探讨PTEN在表没食子儿茶素没食子酸酯（EGCG）增强维甲酸（ATRA）诱导的急性早幼粒细胞白血病（APL）细胞分化中的作用。我们分别将ATRA、EGCG及两种药物联合应用于NB4与HL-60细胞72 h,从形态学上观察细胞核的变化;Western blotting检测PTEN及髓系分化标志CD11b的表达;免疫荧光检测PTEN的入核情况;CCK-8检测细胞增殖率。结果显示,ATRA与EGCG联合作用于NB4与HL-60细胞后,PTEN与CD11b的表达较单独应用ATRA时增加。PTEN特异性抑制剂SF1670作用于NB4后,细胞分化明显减少;PI3K抑制剂LY294002作用后,PTEN及CD11b表达增加。这些均表明EGCG能够增强ATRA诱导APL细胞株的分化作用,且这种促进作用与PTEN的增加是相关的。This study aimed to investigate the effects of PTEN in EGCG enhanced ATRA induced differentiation in acute promyelocytic leukemia cells.After treated with ATRA and EGCG in combination for 72 h,morphological changes of the nucleus were observed in NB4 and HL-60 cells the expression of PTEN and myeloid differentiation markers CD11 b were detected by Western blotting;the level and redistribution of PTEN was examined using Indirect immunofluorescence assay;CCK-8 assay was used to examine the proliferation.We consistently observed that the increased expression of PTEN was closely related to the CD11 b expression in ATRA and the combined treatment.PTEN inhibitor SF1670,inhibited the expression of PTEN and decreased the level of CD11 b;PI3 K inhibitor LY294002,the level of PTEN and CD11 b were increased.These results suggest that EGCG can promotes ATRA-induced maturation of acute promyelocytic leukemia cells via PTEN.

关 键 词：PTEN EGCG 联合治疗 分化 

分 类 号：R733.7[医药卫生—肿瘤]