Interaction between human telomeric G-quadruplexes characterized by single molecule magnetic tweezers  

作　　者：Yi-Zhou Wang Xi-Miao Hou Hai-Peng Ju Xue Xiao Xu-Guang Xi Shuo-Xing Dou Peng-Ye Wang Wei Li 王一舟;侯锡苗;车海鹏;肖雪;奚绪光;窦硕星;王鹏业;李伟(Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics,Chinese Academy of Sciences;School of Physical Sciences, University of Chinese Academy of Sciences;School of Life Sciences, Northwest A & F University)

机构地区：[1]Key Laboratory of Soft Matter Physics, Beijing National Laboratory for Condensed Matter Physics, Institute of Physics, Chinese Academy of Sciences, Beijing 100190, China [2]School of Life Sciences, North west A ＆ F University, Yangling 712100, China [3]School of Physical Sciences, University of Chinese Academy of Sciences, Beijing 100049, China

出　　处：《Chinese Physics B》2018年第6期543-553,共11页中国物理B（英文版）

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.11474346 and 11774407);the Key Research Program of Frontier Sciences,Chinese Academy of Sciences(Grant No.QYZDB-SSW-SLH045);the National Key Research and Development Program,China(Grant No.2016YFA0301500)

摘　　要：Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K~＋buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.Human telomeric G-quadruplex plays a crucial role in regulating the genome stability. Despite extensive studies on structures and kinetics of monomeric G-quadruplex, the interaction between G-quadruplexes is still in debate. In this work,we employ magnetic tweezers to investigate the folding and unfolding kinetics of two contiguous G-quadruplexes in 100-mM K~＋buffer. The interaction between G-quadruplexes and the consequent effect on the kinetics of G-quadruplex are revealed. The linker sequence between G-quadruplexes is further found to play an important role in the interaction between two G-quadruplexes. Our results provide a high-resolution insight into kinetics of multimeric G-quadruplexes and genome stability.

关 键 词：BIOPHYSICS G-QUADRUPLEXES single-molecule studies DNA structures 

分 类 号：O4[理学—物理]