高效液相色谱法测定花生酱中黄曲霉毒素B_1结果不确定度的评定  

Uncertainty evaluation of the determination of Aflatoxin B_1 in peanut butter by high performance liquid chromatography

作　　者：肖利龙[1] 花锦 XIAO Li-long;HUA Jin(Taiyuan College, Taiyuan, Shanxi 030032, China;Taiyuan Institute of Quality Inspection Standards and Metrology, Taiyuan, Shanxi 030032, China)

机构地区：[1]太原学院,山西太原030032 [2]山西出入境检验检疫局检验检疫技术中心,山西太原030032

出　　处：《食品与机械》2018年第3期79-81,150,共4页Food and Machinery

摘　　要：结合CNAS—GL06:2006《化学分析中不确定度的评估指南》和JJF 1059.1—2012《测量不确定度评定与表示》中规定的基本程序,分析测量不确定性的来源,计算测量结果的相对扩展不确定度。结果显示花生酱中黄曲霉毒素B1的含量为25.12μg/kg,相对扩展不确定度为2.1%,测定结果可表示为X=(25.12±0.528)μg/kg,k=2。在影响检测结果的6个不确定度分量中,样品前处理过程和标准工作液配制引入的不确定度对结果影响较大。可通过减少这些不确定度分量以提高测量结果的质量和准确度。In order to evaluate the uncertainty of determination of Aflatoxin B1 in peanut butter by high performance liquid chromatography.According to the uncertainty assessment described in CNAS—GL06-2006 Guidance on evaluation the uncertainty in chemical analysis and JJF 1059.1—2012 Evaluation and expression of uncertainty in measurement,the components of uncertainty in the measuring process were studied and analyzed,and the related expanded uncertainty was calculated and evaluated.The results of method showed the content of Aflatoxin B1 in peanut butter was 25.12μg/kg,the related expanded uncertainty was 2.1%,and the determination result was X=（25.12±0.528）μg/kg,k=2.Among the 6 factors of uncertainty,the content of targeted ions from liners fitting and sample preparation was the main factors which influenced the results mostly.Through reducing the amounts of these factors,the quality of the determination results can be increased.It could improve the accuracy of measurements in routine tests.

关 键 词：黄曲霉毒素B1 花生酱 高效液相色谱法 不确定度 

分 类 号：O657.72[理学—分析化学] TS229[理学—化学]