Identification and Evaluation of a Panel of Ginsenosides from Different Red Ginseng Extracts with Nootropic Effect  

作　　者：DAI Yulin ZHANG Ying ZHAO Xu JEON Youjin ZHENG Fei MA Li YUE Hao 

机构地区：[1]Jilin Ginseng Academy, Changchun University of Chinese Medicine, Changchun 130117, P. R. China [2]Department of Marine Life Science, Jeju National University, Jeju 690-756, Republic of Korea [3]Department of Hepatology, The First Hospital of Jilin University, Changchun 130021, P. R. China [4]Institute of Mass Spectrometer and Atmospheric Environment, Jinan University, Guangzhou 510632, P. R. China

出　　处：《Chemical Research in Chinese Universities》2018年第3期375-381,共7页高等学校化学研究（英文版）

摘　　要：Red ginseng has been gradually discovered to have pharmacological and physiological effects. It is well known that the most important bioactive components of ginseng are ginsenosides. The nootropic effect of ginsenosides from nine different red ginseng extracts was evaluated here. Nine groups of mice were perfused with different concentrations of nine red ginseng extracts, respectively, and two groups of mice with distilled water. The nootropic effect of ginsenosides on mice was evaluated with behavior tests and a biochemical indicator study. The extracts were identified by rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry（RRLC-Q-TOF-MS）. Furthermore, principal component analysis（PCA） was used to analyze the contribution of chemical components from different ginseng groups. The extracts with the most and the weakest effective nootropic were found. It is notable that extract processing is a very important factor to decide pharmacological functions of ginseng extracts. As a conclusion, the most effective extract method for ginsenosides has been found. A panel of 13 ginsenosides has been screened out as chemical markers with nootropic effect, which include high level ginsenosides Ra0, Rb1, Rc, Rb2, Rb3, Re, Rd, and Rgl and low level ginsenosides mRb1, mRc, mRb2, mRd, and F2. Low level ginsenosides were first time to be discovered as possible nootropic compounds. This method may shed light on fast discovery of bioactive compounds of medicinal plants with low level compounds.Red ginseng has been gradually discovered to have pharmacological and physiological effects. It is well known that the most important bioactive components of ginseng are ginsenosides. The nootropic effect of ginsenosides from nine different red ginseng extracts was evaluated here. Nine groups of mice were perfused with different concentrations of nine red ginseng extracts, respectively, and two groups of mice with distilled water. The nootropic effect of ginsenosides on mice was evaluated with behavior tests and a biochemical indicator study. The extracts were identified by rapid resolution liquid chromatography coupled with quadrupole-time-of-flight mass spectrometry（RRLC-Q-TOF-MS）. Furthermore, principal component analysis（PCA） was used to analyze the contribution of chemical components from different ginseng groups. The extracts with the most and the weakest effective nootropic were found. It is notable that extract processing is a very important factor to decide pharmacological functions of ginseng extracts. As a conclusion, the most effective extract method for ginsenosides has been found. A panel of 13 ginsenosides has been screened out as chemical markers with nootropic effect, which include high level ginsenosides Ra0, Rb1, Rc, Rb2, Rb3, Re, Rd, and Rgl and low level ginsenosides mRb1, mRc, mRb2, mRd, and F2. Low level ginsenosides were first time to be discovered as possible nootropic compounds. This method may shed light on fast discovery of bioactive compounds of medicinal plants with low level compounds.

关 键 词：Red ginseng GINSENOSIDE Nootropic effect Mass spectrometry Behavior test 

分 类 号：O629.13[理学—有机化学] TV641.43[理学—化学]