利用12S rRNA基因对四川地区7种牛肉干进行真伪鉴别  

Identification of Seven Species of Commercial Beef Jerky Based on Mitochondrial 12S rRNA Gene

作　　者：吴周林 赵莉 王健蓉 徐弘扬 左玲 董婷婷 罗海艳 赵吉连 Wu Zhoulin;Zhao Li;Wang Jianrong;Xu Hongyang;Zuo ling;Dong Tingting;Luo Haiyan;Zhao Jilian(College of Agronomy, Siehuan Minzu College, Kangding, Siehuan 626001, China)

机构地区：[1]四川民族学院农学院,四川康定626001

出　　处：《当代畜牧》2018年第4期61-63,共3页Contemporary Animal Husbandry

基　　金：四川省大学生创新训练项目(201611661014);四川省教育厅科研项目(18ZB0452);四川民族学院大学生创新创业训练计划项目(201711661016)

摘　　要：笔者针对牦牛线粒体12S r RNA基因设计特异性引物,以市场销售的牦牛肉干为研究对象,建立牦牛肉干中肉源性成分的PCR鉴定方法,并用该方法对四川地区销售的7种牛肉干进行检测。结果表明,所检测样品在440 bp处出现预期条带,检出率为100%。将PCR产物经测序后采用DNAMAN进行序列比对分析,并通过MEGA 5.10软件构建进化树,6份牦牛肉干样本中6份与标注吻合,1份黄牛肉干检测出是牦牛肉。试验表明,牦牛线粒体12S r RNA基因序列分析可用于牛肉干的鉴别,市场销售的牛肉干存在掺假现象。This study was conducted to authenticate the species of beef jerky. We selected seven kinds of commercial beef jerky from a market in Kangding, and selected a pair of specific primers to amplification the mitochondrial 12S rRNA. There is a light band near the the position of 440 bp in 1.5% （w/v） agarose gel among all the samples and that was in line with expectations. The DNA sequences were assembled and analyzed with the DNAstar program and the phylogenetic tree was established by MEGA 5.10. Six yak jerky was in accor- dance with the labels, but one beef jerky was not and might be instead of yak meat.

关 键 词：牛肉干 鉴别 12SRRNA基因 

分 类 号：R446[医药卫生—诊断学]