miR-145在非小细胞肺癌中的表达及临床病理意义  

Expression of mi R-145 in Non-small Cell Lung Cancer and Its Clinicopathological Significance

作　　者：王瑞娟[1] 郑艳华[1] 许杨[1] 霍苗[1] 邳建军 王萍[1] 张建中[1] WANG Rui-juan;ZHENG Yan-hua;XU Yang(The 306th Hospital of PLA ,Beijing 100101 ,China)

机构地区：[1]中国人民解放军第306医院

出　　处：《肿瘤学杂志》2018年第5期425-428,共4页Journal of Chinese Oncology

基　　金：首都医学发展科研基金重点支持项目(SF2011-5005-01)

摘　　要：[目的]探讨mi R-145在配对的非小细胞肺癌（non-small cell lung cancer,NSCLC）患者肿瘤组织和癌旁组织中的表达情况,分析其与临床病理因素和预后的关系。[方法]应用实时定量聚合酶链反应（q-RT-PCR）检测mi R-145在配对的NSCLC患者肿瘤组织和癌旁组织中的表达水平。[结果]mi R-145在NSCLC患者肿瘤组织中的表达水平明显低于癌旁组织（P〈0.001）。mi R-145诊断NSCLC的ROC曲线下面积为0.895。mi R-145在Ⅲ~Ⅳ期和中低分化的NSCLC患者肿瘤组织中的表达水平明显低于Ⅰ~Ⅱ期和高分化的肿瘤组织（P〈0.05）。mi R-145低表达组的总生存率明显低于mi R-145高表达组（P〈0.05）。[结论 ]mi R-145在NSCLC组织中的表达下调,在NSCLC的发生中可能发挥抑癌基因的作用,可能是NSCLC的诊断及预后标志物之一。[Objective] To explore the expression of mi R-145 in non-small cell lung cancer（NSCLC） tissue and its adjacent tissue and its clinicopathological significance. [Methods] Expression level of mi R-145 was detected in tumor tissue and its adjacent tissue by quantitative real-time PCR（q RT-PCR） from 62 patients with NSCLC. [Results] The expression of mi R-145 in tumor tissue was significantly lower than that in the adjacent tissue（P0.001）. The area under the ROC curves（AUC） for mi R-145 was 0.895. The expression of mi R-145 was significantly lower in stage Ⅲ~Ⅳ and in moderate-poorly differentiated tumor tissue than that in stage Ⅰ ~ Ⅱ and in well-differentiated tumor tissue（P〈0.05）. The overall survival rate in patients with low expression mi R-145 was significantly lower than those with high expression（P〈0.05）. [Conclusion] The expression of mi R-145 is down-regulated in NSCLC. Mi R-145 might function as anti-oncogene and be one of the potential biomarkers for the diagnosis and prognosis in patients with NSCLC.

关 键 词：非小细胞肺癌 MIR-145 组织 诊断 预后 

分 类 号：R734.2[医药卫生—肿瘤]