宫颈癌宫颈脱落细胞中miR-21的表达及其诊断价值的探讨  

The Diagnostic value of miR-21 in cervical exfoliated cells of cervical cancer patients

作　　者：支亚丽 张诗蒙[1] 栗俊杰[1] 谭志容 李树隆 庄严[1] ZHI Ya-li;ZHANG Shi-meng;LI Jun-jie(Shenzhen Shekou People ＇ s Hospital ,Shenzhen 518067 ,China)

机构地区：[1]深圳市蛇口人民医院,广东深圳518067

出　　处：《中国实验诊断学》2018年第5期798-800,共3页Chinese Journal of Laboratory Diagnosis

基　　金：深圳市知识创新计划基础研究项目(项目编号:JCYJ20150403093555010)

摘　　要：目的比较正常组与宫颈上皮内瘤变组、宫颈癌组宫颈脱落细胞中miR-21的相对表达水平是否在各组间存在明显差异。方法收集宫颈脱落细胞标本,正常对照组与宫颈上皮内瘤变组、宫颈癌组各30例,以U6为内参miRNA,用SYBR Green荧光定量PCR法测定各组中miR-21的表达水平,并评估miR-21对宫颈癌的诊断效能。结果宫颈上皮内瘤变组、宫颈癌组宫颈脱落细胞中miR-21的表达上调。miR-21区别正常组与宫颈上皮内瘤样病变组的ROC曲线面积为0.87(95%CI:0.7935-0.9621;P<0.000 1),miR-21区别正常组与宫颈癌组的ROC曲线面积为0.92(95%CI:0.8590-0.9899;P<0.000 1)。结论宫颈粘液脱落细胞中的miR-21对宫颈癌筛查诊断有潜在临床应用价值。Objective The deregulation of miR-21 was described in multiple tumors,so miR-21 could be a potential biomarker of different cancers.The aim of this study was to compare the relative expression levels of miR-21 in cervical exfoliated cells between normal cervix group,cervical intraepithelial neoplasia（CIN）group and cervical cancer group.Methods Thirty cervical exfoliated cells in each group were collected for microRNA detection.The expression level of miR-21 was detected by SYBR Green Real-time PCR.U6 was used as reference microRNA.The diagnostic value was analyzed.Results Higher level of miR-21 was observed in CIN group and cervical cancer group than normal cervix group.MiR-21 could discriminate nomal group and CIN group,the area under the ROC curve was 0.87,（95%CI：0.7935-0.9621,P〈0.0001）and for discriminating normal group and cervical cancer group,the area under the ROC curve was 0.92（95%CI：0.8590-0.9899;P〈0.0001）.Conclusion MiR-21 could be a potential biomarker for the diagnosis of cervical cancer.

关 键 词：宫颈癌 宫颈上皮内瘤变 MIR-21 

分 类 号：R737.33[医药卫生—肿瘤]