实时荧光定量PCR法检测EB病毒DNA在儿童EB病毒感染中的应用  

Application of EB-DNA detection based on Real time PCR in Children with EB Virus Infection

作　　者：宋予娟[1] 耿岚 王华[1] 许冰 南黎[1] 魏巍[1] SONG Yu-juan;GENG Lan;WANG Hua(The First Hospital of Xinxiang, Henan Xinxiang 45300)

机构地区：[1]新乡市第一人民医院医学检验科,河南新乡453000 [2]新乡市传染病医院防保科,河南新乡453000

出　　处：《医学检验与临床》2018年第4期18-20,共3页Medical Laboratory Science and Clinics

摘　　要：目的：探讨EB病毒（EB Virus,EBV）DNA检测在儿童EB病毒感染诊断中的应用,为临床提供早期诊断准确治疗.方法：采用实时荧光定量PCR法（Real time PCR,RT-PCR）对已确诊的50例感染EBV的儿童（A组）进行外周血EBV-DNA检测.并同时检测患儿的白细胞（White Blood Cell,WBC）、异常淋巴细胞、超敏C反应蛋白（hypersensitive C-reactive protein,hs-CRP）、谷丙转氨酶（Alanine aminotransferase,ALT）、谷草转氨酶（Aspartate aminotransferase,AST）、肌酸激酶（Creatine kinase,CK）MB型CK（CK-MB）等实验室检测的相关数据.同时分析同期同年龄段的50例（B组）健康体检儿童的WBC、 异常淋巴细胞、hs-CRP、ALT、AST、CK、CK-MB等实验室检测的相关数据.结果：A组WBC、 异常淋巴细胞、ALT、AST、CK、CK-MB的检测水平明显高于B组（P〈0.05）;EBV-DNA阳性检出率也明显高于B组（P〈0.05）.结论：EB病毒感染儿童临床表现复杂,EBV-DNA检测可用于EBV感染相关疾病的早期诊断及病情的评估,值得应用于临床推广.Objective： The study aims to explore the application ofEBV DNA in the diagnosis of Epstein-Barr vires in children and to provide early diagnosis and accurate treatment for clinical. Methods： Fluorescent quantitative PCR was used to detect EB-DNA in peripheral blood of 50 confirmed children with EB disease （group A）. At the same time, the related data of laboratory tests such as WBC, abnormal lymphocytes （hs-CRP）, ALT, AST, CK, CK-MB were detected. Results： The levels ofWBC, hs-CRP, ALT, AST, CK and CK-MB in group A were significantly higher than those in group B （P〈0.05）. The positive rate of EB-DNA of group A was also significantly higher than that of group B （P〈0.05）. Conclusion： The clinical manifestations of children with Epstein-Barr virus infection are complicated. The detection ofEBV DNA could be used in the early diagnosis and assessment of EBV-related diseases, which is worthy of clinical application.

关 键 词：EB病毒DNA 实时荧光定量PCR EB病毒 传染性单核细胞增多症 异常淋巴细胞 

分 类 号：R733.7[医药卫生—肿瘤]